FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15110
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA782851.806750496360024No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA190412.600926538716081No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA13198.729318332230312No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATCACAGAGACCAAGTCTCTG1801.1912640635340834No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATCACAGAGACCAAGTCTCTG1240.8206485771012575No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG890.5890138980807412No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG870.5757776307081403No Hit
GTGCTGTCTCTCTTTGATGGATCTCAGTGCGGTGATGGTAGCCTTTCTGC650.43017868960953015No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG640.42356055592322966No Hit
GGTGTTTGGGATGGAATGGTGGAGCGCTTGTTGTAGTGCGCCAGGCGGGA630.4169424222369292No Hit
CTCCAAGTAACGGTGCTGTCTGCTAGCTGGTGCAGGGCTCCTGGTGCTGA620.41032428855062875No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTCACAGAGA620.41032428855062875No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG570.3772336201191264No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATCACAGA570.3772336201191264No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC560.37061548643282594No Hit
GTGCTGTCTCTCTTTGATGGAATCTCTGTGTCCTGGGGCATCAATGATAG560.37061548643282594No Hit
GTGCTGTCTCTCTTTGATGGAATCATACTATATGTTGAGTTCTGTGCTTC550.3639973527465255No Hit
GTGCTGTCTCTCTTTGATGGAATCCGCTCTTGCTTCTCTTTCTTGGGTGT470.3110522832561218No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCGGGTGGTTCGCAGAAGCAGCA420.27796161482461945No Hit
GTGCTGTCTCTCTTTGATGGACTTGCCTCCTTCACTATAGGCAATATTCC420.27796161482461945No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATCACAGAGACCAAGTCTCTG400.26472534745201853No Hit
GTGCCTCCTTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTA380.25148908007941756No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC340.22501654533421572No Hit
GTGCTGTCTCTCTTTGATGGATATGAAGCACCGCCAGGTCCTTTGAGTTT330.21839841164791532No Hit
GTGCTGTCTCTCTTTGATGGAATCAAGTGCTGCCTGATCAATGGTGATCA330.21839841164791532No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC330.21839841164791532No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG290.19192587690271343No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA280.18530774321641297No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG260.17207147584381205No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG250.1654533421575116No Hit
GTGCTGTCTCTCTTTGATGGAATCAGTGGTATCAACGCAGAGTACATGGG250.1654533421575116Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGAAGTAGACATGGGAGACCTTTCATTTTGTT240.1588352084712111No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGTA230.15221707478491067No Hit
GTGCTGTCTCTCTTTGATGGAATCTCTTTTGTCATCTCTGTGCCTCCTTC210.13898080741230973No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA210.13898080741230973No Hit
GGGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG210.13898080741230973No Hit
GTGCTGTCTCTCTTTGATGGAATCAGGTCGGCTGCTATGAGGTTGAAGCC200.13236267372600927No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCGGCA200.13236267372600927No Hit
GTGCTGTCTCTCTTTGATGGATTTTGCCGTGTCTGTGTAGAAAGAAGTAG190.12574454003970878No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA190.12574454003970878No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT180.11912640635340835No Hit
GTGCTGTCTCTCTTTATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC170.11250827266710786No Hit
GGTGTTTGGGATGGAATGGCGATCCACGCAGGTGGTTCGCAGAAGCAGCA170.11250827266710786No Hit
GTGCTGTCTCTCTTTGATGGAAGCAGTGGTATCAACGCAGAGTACATGGG170.11250827266710786Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAAAAGTTCTCAGGGATGTCCTCAGCAACCCTGTGATGATTGATGCTCA170.11250827266710786No Hit
GTGCTGTCTCTCTTTGATGGAATGTGGTGGGGGTCTCTTGGGCAGGGAAT160.10589013898080742No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA160.10589013898080742No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)