FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13557
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT675649.83403407833592No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA175812.96747067935384No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA4723.481596223353249No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGT3312.441543114258317No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC2071.5268864793095818No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC1991.4678763738290181No Hit
CAACCAAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGT1120.8261414767278896No Hit
CAACCAAAAGAGAAGGAGGCACACCTTATCCTACAACCTAGGGAGTGGCA1090.8040126871726783No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA1090.8040126871726783No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1060.781883897617467Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCATGTT960.7081212657667626No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC700.516338422954931No Hit
CAACCAAAAGAGAAGGAGGCACGAAAGGCAGGTGAGGCCCGGAAGCAGGC690.5089621597698606No Hit
CAACCAAAAGAGAAGGAGGCACAGGAAAATATCCTCCTGCTAGTCTGCAT620.4573283174743674No Hit
CAACCAAAAGAGAAGGAGGCACTTACACATTCCTAGTGTCCAATTCTGAC590.4351995279191561No Hit
CAACCAAAAGAGAAGGAGGCACCACAAAAAGCTGAGGCCCAATGTAAGGA520.38356568562366306No Hit
CAACCAAAAGAGAAGGAGGCACGTCTTACATGGGCAGAGAAGGAGGAAGA510.3761894224385926No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT500.36881315925352215No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT420.3098030537729586No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC390.2876742642177473Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT380.2802980010326769No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA370.2729217378476064No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT370.2729217378476064No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGT300.22128789555211328No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA300.22128789555211328Clontech Universal Primer Mix Long (96% over 26bp)
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCATGTTGTAGA280.2065353691819724No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGAAC280.2065353691819724No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT270.19915910599690198No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA270.19915910599690198No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT270.19915910599690198No Hit
TCCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT250.18440657962676107No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA240.17703031644169065No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT230.1696540532566202No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA230.1696540532566202No Hit
CAACCAAAAGAGAAGGAGGCACAAGCACTCCTCTGAGTCGCCAGGGAGGG230.1696540532566202No Hit
CAACCAAAAGAGAAGGAGGCACAGTTGGATGATAGGACAAAAATCACTGA220.16227779007154974No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT220.16227779007154974No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA210.1549015268864793No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA210.1549015268864793No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATGTTG200.14752526370140887No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC180.13277273733126796Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGA180.13277273733126796No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT170.12539647414619753No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT170.12539647414619753No Hit
CAACCAAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTCAGTGTA170.12539647414619753No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCCTCACTTACTCT160.1180202109611271No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT160.1180202109611271No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA160.1180202109611271No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT160.1180202109611271No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGACCCTGGGCTTCACTTACTCT160.1180202109611271No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCATGTT160.1180202109611271No Hit
CAACCAAAAGAGAAGGAGGCACCCACAGTGTCCCCAGCAGTGGCACAGAG150.11064394777605664No Hit
CAACCAAAAGAGAAGGAGGCACGTGGCAGTGAAGGGAAATCATTGTGATG150.11064394777605664No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACCCT150.11064394777605664No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT140.1032676845909862No Hit
CAACCAAAAGAGAAGGAGGCAGAGAGAGATAAGTGGGAGGTGCTACATGC140.1032676845909862No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGTCCTGGGCTTCACTTACTCT140.1032676845909862No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)