FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6061
Total Bases877.7 kbp
Sequences flagged as poor quality0
Sequence length135-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA248641.01633393829401No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA3105.11466754660947Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA2964.8836825606335585No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGT1923.1677940933839297No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA1883.101798383105098No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC1412.3263487873288238No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1352.227355221910576Clontech Universal Primer Mix Long (96% over 26bp)
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC1121.8478798878072924No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC961.5838970466919648Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGT621.022933509321894No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCATGTT470.7754495957762745No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA460.7589506682065665No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA450.7424517406368586No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC410.6764560303580267No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC380.6269592476489028Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCCACTGCACCTGCTGAAGTTCCATCAAA340.5609635373700709No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA330.5444646098003629No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGAAC310.511466754660947No Hit
GCATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG300.49496782709123904Clontech Universal Primer Mix Long (96% over 26bp)
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA280.4619699719518231No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCATGTTGTAGA260.42897211681240716No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA220.3629764065335753No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGA210.3464774789638673No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA200.3299785513941594No Hit
GGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA190.3134796238244514No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC190.3134796238244514Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG190.3134796238244514Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGT180.29698069625474344No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATGTTG170.28048176868503544No Hit
GGGCAAAGGGGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA170.28048176868503544No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA170.28048176868503544No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA170.28048176868503544No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA160.2639828411153275No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA150.24748391354561952Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCAATCATCACAGGGTTGGCCGACCTGATGTCAACCTGCTTGGGTGA140.23098498597591155No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC140.23098498597591155No Hit
GGGCAAAGGGTGAAGGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA140.23098498597591155No Hit
CAACCAAAAGAGAAGGAGGCACAGAAGTCCATCAAAGAGAGACAGCACCC130.21448605840620358No Hit
CAACCAAAAGAGAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGTA130.21448605840620358No Hit
CAACCAAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTCAGTGTA120.1979871308364956No Hit
CAACCAAAAGAGAAGGAGGCACGATTCCATCAAAGAGAGACAGCACCCAT100.1649892756970797No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCATGTT100.1649892756970797No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG90.14849034812737172No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAGTGAAAGGAGGCA90.14849034812737172No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG90.14849034812737172No Hit
GCATCAATCATCACAGGGTTGGCCGACCTGATGTGAACCTGCTTGGGTGA90.14849034812737172No Hit
TCCTATCAGTGGCCGCAAAGCAGTGGTATCAACGCAGAGTACATGGGAAG90.14849034812737172Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAGCAAAATGAAAGGAGGCA80.13199142055766375No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAGTGAACAAAATGAAAGGAGGCA80.13199142055766375No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT80.13199142055766375No Hit
GCATCAATCATCACAGGGTTGGCCGACCTGATGTAAACCTGCTTGGGTGA80.13199142055766375No Hit
CAACCAAAAGAGAAGGAGGCAGCACCCATGTTGTAGAACCATGTCGTCAG80.13199142055766375No Hit
GGGCAAAGGGTGAAAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA70.11549249298795577No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCCATGT70.11549249298795577No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGGACAAAATGAAAGGAGGCA70.11549249298795577No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAGAGGAGGCA70.11549249298795577No Hit
TCCTATCAGTGGCCGCACCATTCCATCCCAAACACCCCATGTTGTAGAAC70.11549249298795577No Hit
CAACCAAAAGAGAAGGAGGCAGAGAGAAAGGAGAGGTAAAAATGTTGTAC70.11549249298795577No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)