FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5189
Total Bases752.1 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA198538.25399884370784No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGT89417.22875313162459No Hit
CAACCAAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGT1302.505299672383889No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1252.4089419926768163Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCATGTT1042.004239737907111No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC1021.965696666024282No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC851.638080555020235No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA811.560994411254577No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC551.0599344767777992Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG460.8864906533050684No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA440.8479475814222392No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA390.7515899017151667Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCATGTT310.5974176141838505No Hit
CAACCAAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTCAGTGTA310.5974176141838505No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGAAC300.5781460782424359No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC300.5781460782424359No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATAAAAGA290.5588745423010214No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC250.4817883985353632Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATGTTG240.46251686259394875No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCATGTTGTAGA230.4432453266525342No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCAACAAAGA200.3854307188282906No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA180.34688764694546154Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG180.34688764694546154No Hit
CAACAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG180.34688764694546154No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGA180.34688764694546154No Hit
CAACCAAAAGAGAAGGGGGCACCATCAAAGAGAGACAGCACCATCAAAGA170.32761611100404703No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCATGT160.30834457506263246No Hit
CACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG160.30834457506263246No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT160.30834457506263246No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCAAAGA150.28907303912121796No Hit
CAACCAAAAGAGAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGTA150.28907303912121796No Hit
AACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG150.28907303912121796No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGT140.26980150317980345No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGTA130.2505299672383889No Hit
CAACAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGTA120.23125843129697438No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCATAGA100.1927153594141453No Hit
GCATCAATCATCACAGGGTTGGAAGCAGTGGTATCAACGCAGAGTACATG90.17344382347273077Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTCAGTGTAG90.17344382347273077No Hit
GCATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG90.17344382347273077Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGTA90.17344382347273077No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCGCCATCAAAGA80.15417228753131623No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCCATCAAA80.15417228753131623No Hit
GCATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG80.15417228753131623Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCATCAAA70.13490075158990172No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGTACCATCAAAGA70.13490075158990172No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAGGAGAGACAGCACCATCAAAGA70.13490075158990172No Hit
CAACCAAAAGAAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG70.13490075158990172No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGCCAGCACCATCAAAGA60.11562921564848719No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC60.11562921564848719Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCAATCATCAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTC60.11562921564848719No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATGAAAGA60.11562921564848719No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACGGCACCATCAAAGA60.11562921564848719No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGGCAGCACCATCAAAGA60.11562921564848719No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCCATGT60.11562921564848719No Hit
GGGCAAAGGGTGAAGAGAAAGCACCGTTACTTGGAGCCATGTTGTAGAAC60.11562921564848719No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAATGA60.11562921564848719No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)