FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19422
Total Bases2.8 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA1194361.49212233549582No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATCACAGAGACCAAGTCTCTG4292.208835341365462No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATCACAGAGACCAAGTCTCTG3811.9616929255483473No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA2471.2717536813922357No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA2271.1687776748017713No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA2041.050355267222737No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC1610.8289568530532386No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC1530.7877664504170527No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA1510.7774688497580065No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTCACAGAGA1280.6590464421789722No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATCACAGA1120.5766656369066008No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATCACAGAGACCAAGTCTCTG1050.5406240345999382No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA940.48398723097518276No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC840.4324992276799506No Hit
GTGCCTCCTTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTA700.36041602306662546No Hit
GTGCTGTCTCTCTTTGATGGAAAGAGGTAGACATGGGACACTTTTCATTT650.33467202141900937No Hit
CTCCAAGAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA630.3243744207599629No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTCACAGAGACCA510.26258881680568424No Hit
GTGCTGTCTCTCTTTGATGGAATCAGATTTTCTGATCACGTAGCCCTTTT500.25744001647616105No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCAT490.25229121614663785No Hit
GTGCTGTCTCTCTTTGATGGAATGAGGTAGACATGGGACACTTTTCATTT480.2471424158171146No Hit
GTGCTGTCTCTCTTTGATGGATAGGAAGCTTGTGATTTTTGCACATTGAT460.23684481515806816No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTCACAGAGACCA440.22654721449902174No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCGGGGCTCCTGGTGCTGA430.22139841416949851No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTCACAGAGACCAAGTCTCT430.22139841416949851No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTCACAGAGACCAAG430.22139841416949851No Hit
GTGCTGTCTCTCTTTGATGGAATCCATCCGTCGTGGTGCAGGACTATTAA410.21110081351045207No Hit
CTCCAAGTAAGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA400.20595201318092884No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA390.2008032128514056No Hit
CCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA380.19565441252188243No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT360.18535681186283595No Hit
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTCACAGAG360.18535681186283595No Hit
GTGCTGTCTCTCTTTGATGGAATCGGTCTTTTATATCCATTTGGCGTCTA340.17505921120378953No Hit
CTCCAAGTAACGGTGCTGCCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA320.16476161054474306No Hit
CTCCAAGTAACGGGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA320.16476161054474306No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGACTCCTGGTGCTGA310.15961281021521986No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC310.15961281021521986No Hit
CTCCAAGTAACGGTCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA300.15446400988569664No Hit
GCAAAAGTTCTCAGGGATGTCCGCATTCGCACTTCCCGTGCCTCCTTCTC290.14931520955617342No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGTAGGGCTCCTGGTGCTGA290.14931520955617342No Hit
GGTGTTTGGGATGGAATGGTATCAACGCAGAGTACATGGGAAGCAGTGGT280.1441664092266502Clontech SMARTer II A Oligonucleotide (95% over 21bp)
GTGCTGTCTCTCTTTGATGGAATCTGGGCCGTGTCTCAGTCCCAGTGTGG260.13386880856760375No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGTTGA250.12872000823808052No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT250.12872000823808052Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGAATCCTGTCAATACTCCCTCAAACATCTCT240.1235712079085573No Hit
CTCCAAGTAACGGTGCTGTCTGTTGGCTGGTGCAGGGCTCCTGGTGCTGA220.11327360724951087No Hit
GTGCTGTCTCTCTTTGATGGAGGGTCTTTAACCTGGACGTTTAGAGCATC220.11327360724951087No Hit
GTGCTGTCTCTCTTTGATGGAATGTTCTCGTCGTCTTTCTTCTCCTCGCC220.11327360724951087No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT200.10297600659046442Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAAGTAACGGTGCTGTCTGCTGGCTAGTGCAGGGCTCCTGGTGCTGA200.10297600659046442No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)