FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4100
Total Bases594 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA178543.53658536585366No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC2686.536585365853659No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTCACAGAGA1994.853658536585366No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA1593.8780487804878048No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC1573.8292682926829267No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT1142.780487804878049No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT340.8292682926829269Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT340.8292682926829269No Hit
GTGCTGTCTCTCTTTGATGGAATCAGTTCCGAAAACCAACAAAATAGAAC320.7804878048780488No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG280.6829268292682927No Hit
GTGCCTCCTTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTA270.6585365853658537No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT260.6341463414634146Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCCAAGTAACGGTGCGGCCACTGATAGGATCACAGAGACCAAGTCTCTG240.5853658536585366No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG240.5853658536585366No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC180.4390243902439025No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG170.41463414634146345No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATCACAGAGACCAAGTCTCTG160.3902439024390244No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG150.3658536585365854No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC140.34146341463414637No Hit
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTCACAGAG130.3170731707317073No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCAGTCTCTC120.2926829268292683No Hit
GTGCTGTCTCTCTTTGATGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC120.2926829268292683No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC120.2926829268292683No Hit
GTGCTGTCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAG120.2926829268292683No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC110.2682926829268293Clontech SMART CDS Primer II A (95% over 24bp)
GTGCTGTCTCTCTTTGATGGAATCGAAAGCGGGGAAAGGTGGGGAAAAGA100.24390243902439024No Hit
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTCACAGA100.24390243902439024No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCGGTCTCTC100.24390243902439024No Hit
GTGCTGTCTCTCTTTGATGGAATCTACCACCCGCTTTGGGCTGCATTCCC90.21951219512195125No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATCACAGA90.21951219512195125No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCCTCC70.17073170731707318No Hit
GTGCTGTCTCTCTTTGATGGAATTTACCACCCGCTTTGGGCTGCATTCCC70.17073170731707318No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG70.17073170731707318No Hit
GCAAAAGTTCTCAGGGATGTCCTGGTCCCGCCAATAGAATCCCAATCCTT70.17073170731707318No Hit
GTGCTGTCTCTCTTTGATGGACCCGTGCCTCCTTCTCTTTTGGTTGTCAC60.14634146341463414No Hit
GTGCTGTCTCTCTTTGATGGACTCAGTTCCGAAAACCAACAAAATAGAAC60.14634146341463414No Hit
GGTGTTTGGGATGGAATGGTGGTCCACGCAGGTGGTTCGCAGAAGCAGCA60.14634146341463414No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCGGGTGGTTCGCAGAAGCAGCA60.14634146341463414No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTCTGCGTTGATACCA60.14634146341463414No Hit
GGTGTTTGGGATGGAATGGTGTTCCACGCAGGTGGTTCGCAGAAGCAGCA60.14634146341463414No Hit
GGGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG60.14634146341463414No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG60.14634146341463414No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTCTCTTTTGGTTGTCACAGAGAC50.12195121951219512No Hit
GTGCTGTCTCTCTTTGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC50.12195121951219512No Hit
GTGCTGTCTCTCTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAGG50.12195121951219512No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATCACAGAGACCAAGTCTCTG50.12195121951219512No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG50.12195121951219512No Hit
GTGCCTCCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAG50.12195121951219512No Hit
GTGCTGTCTCTCTTTGATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC50.12195121951219512No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTCACAG50.12195121951219512No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG50.12195121951219512No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG50.12195121951219512No Hit
GTGCTGTCTCTCTTTGAGGGTCATCCCCAAGCAGCTGCGTTGATACCACT50.12195121951219512No Hit
GTGCTGTCTCTCTTTGATGGACAGGTGCTGTCTCTCTTTGATGGACAGGT50.12195121951219512No Hit
GTGCTGTCTCTCTTTGATGGAGTGCCTCCTTCTCTTTTGGTTGTCACAGA50.12195121951219512No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA50.12195121951219512No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)