FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008148970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008148970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18457
Total Bases2.6 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA1057457.28991710462156No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA7454.036408950533673No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGT4412.389337378772282No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC4052.1942894294847486No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC3902.113019450614943No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT2871.5549655957089452No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2501.3544996478300917Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA2171.1757056943165196No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA1570.8506257788372974No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA1300.7043398168716476No Hit
CAACCAAAAGAGAAGGAGGCACAGCTCATGCTTTCCCGTAAGATGGCCCA1270.6880858210976866No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA1170.6339058351844828No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCATGTT1140.6176518394105218No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC1130.6122338408192014No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA1090.59056184645392No Hit
CAACCAAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGT1050.5688898520886385No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGT860.46594787885355154No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGAAC840.4551118816709107No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCATGTTGTAGA840.4551118816709107No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA820.44427588448827No Hit
GGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA690.3738419028011053No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGA680.36842390420978494No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC650.3521699084358238Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATGTTG600.325079915479222No Hit
GGGCAAAGGGGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA600.325079915479222No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA530.2871539253399794Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA510.2763179281573387No Hit
GGGCAAAGGGTGAAGGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA480.2600639323833776No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA410.222137942244135No Hit
GGGCAAAGGGTGAAAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA350.18962995069621283No Hit
CAACCAAAAGAGAAGGAGGCACACAGAGACACACAAAAACATACAGACAA350.18962995069621283No Hit
GGGCAAAGGGTGAAGAGAAAGGGGGCAAATGAACAAAATGAAAGGAGGCA330.17879395351357208No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT330.17879395351357208No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCATGTT310.16795795633093136No Hit
GGGCAAAGGGTGAGGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA310.16795795633093136No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC310.16795795633093136No Hit
TCCTATCAGTGGCCGCAAAGAGAGCTACTCCATCTACGTGTACAAGGTGC300.162539957739611No Hit
CAACCAAAAGAGAAGGAGGCACAGAATAATCAAGAATGAAGTTAAGAAGC280.15170396055697027No Hit
GGGCAAAGGGTGAAGAGAAAGAGAGCAAATGAACAAAATGAAAGGAGGCA270.14628596196564989No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCG250.13544996478300916No Hit
GGGCAAAGGGTGAAGGGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA250.13544996478300916No Hit
GGGCAAAGGGTGAAGAGAAAGAGGACAAATGAACAAAATGAAAGGAGGCA250.13544996478300916No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA230.12461396760036843No Hit
CAACCAAAAGAGAAGGAGGCACAGAGATGACAAAAGAGATTCCATCAAAG230.12461396760036843No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAGGGAGGCA230.12461396760036843No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGACA220.11919596900904807No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGTAAATGAACAAAATGAAAGGAGGCA210.11377797041772768No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAGAGGAGGCA200.10835997182640732No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCCATGT190.10294197323508696No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGGACAAAATGAAAGGAGGCA190.10294197323508696No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)