FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11614
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA588050.628551747890484No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA133411.486137420354744No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTCACAGAGA3663.1513690373686933No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC3112.677802651971758No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA2902.4969863957292926No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATCACAGAGACCAAGTCTCTG1521.3087652832788015No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT1511.3001549853624936No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATCACAGAGACCAAGTCTCTG1241.06767694162218No Hit
GTGCCTCCTTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTA870.7490959187187877No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC840.723265024969864No Hit
GTGCTGTCTCTCTTTGATGGACAAAACTTTCATGACACGCATGCGCAAAA810.6974341312209402No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG760.6543826416394007No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG630.542448768727398No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG580.49939727914585846No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATCACAGA450.3874634062338557No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC400.3444119166523162No Hit
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTCACAGAG390.3358016187360083No Hit
GTGCTGTCTCTCTTTGATGGAGCTTGTAATGAATTGGGTGATGCCCATTC330.2841398312381609No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA330.2841398312381609No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA300.2583089374892371No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG280.24108834165662132No Hit
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTCACAGA280.24108834165662132No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATCACAGAGACCAAGTCTCTG270.23247804374031344No Hit
GTGCTGTCTCTCTTTGATGGATATGAAGCACCGCCAGGTCCTTTGAGTTT250.2152574479076976No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG250.2152574479076976No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA240.2066471499913897No Hit
GTGCTGTCTCTCTTTGATGGACAAGTCACCCAAGCAGGTTGACATCAGGT230.19803685207508182No Hit
GTGCTGTCTCTCTTTGATGGAAATAAGCAAATGGTAGAAGAAGACCATTT230.19803685207508182No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCACTGCTTCC220.18942655415877388Clontech Universal Primer Mix Long (96% over 25bp)
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT200.1722059583261581Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG190.16359566040985019No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC190.16359566040985019No Hit
GTGCTGTCTCTCTTTGATGGAATCTCCATGAAAGCAAGAGACCCTACCTG170.14637506457723437No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCCTCCTT170.14637506457723437No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG160.13776476666092646No Hit
GTGCTGTCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAC160.13776476666092646No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG160.13776476666092646No Hit
GGTGTTTGGGATGGAATGGTGATCCGCGCAGGTGGTTCGCAGAAGCAGCA150.12915446874461856No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTCACAGAGACCAAGTCTCT150.12915446874461856No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGTAGCA140.12054417082831066No Hit
GGTGTTTGGGATGGAATGGCGATCCACGCAGGTGGTTCGCAGAAGCAGCA140.12054417082831066No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA140.12054417082831066No Hit
GCAAAAGTTCTCAGGGATGTCTCAGCAACTCTGCGTTGATACCACTGCTT140.12054417082831066Clontech SMART CDS Primer II A (95% over 24bp)
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA140.12054417082831066No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT130.11193387291200274No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAGGCAGCA120.10332357499569485No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCGGCA120.10332357499569485No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC120.10332357499569485No Hit
GTGCTGTCTCTCTTTGATGGAATCAGTGGTATCAACGCAGAGTACATGGG120.10332357499569485Clontech SMARTer II A Oligonucleotide (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)