FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11798
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA398433.7684353280217No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA289724.555009323614172No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA5824.933039498220038No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATCACAGAGACCAAGTCTCTG3172.6868960840820475No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC2512.1274792337684354No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATCACAGAGACCAAGTCTCTG2382.0172910662824206No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTCACAGAGA2021.7121546024749956No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT1150.974741481607052No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC980.8306492625868791No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATCACAGA950.8052212239362604No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG570.48313273436175624No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA570.48313273436175624No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATCACAGAGACCAAGTCTCTG560.47465672147821664No Hit
GTGCCTCCTTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTA520.44075266994405826No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA470.39837260552636045No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG470.39837260552636045No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA440.3729445668757417No Hit
GTGCTGTCTCTCTTTGATGGAAAGAAGTAGACATGGGAGACTTTTTATTT430.36446855399220207No Hit
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTCACAGAG370.3136124766909646No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA350.29666045092388543No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTCACAGAGACCAAG340.2881844380403458No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTCACAGAGACCAAGTCTCT330.2797084251568062No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG320.27123241227326667No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTCACAGAGACCA300.25428038650618745No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG280.23732836073910832No Hit
GTGCTGTCTCTCTTTGATGGAATCAGTGGTATCAACGCAGAGTACATGGG260.22037633497202913Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA260.22037633497202913No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG250.21190032208848958No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTCACAGAGACCA240.20342430920494997No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC240.20342430920494997No Hit
CTCCAAGAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA220.18647228343787084No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT210.17799627055433126No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT210.17799627055433126Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTCTCTCTTTGATGGATATGAAGCACCGCCAGGTCCTTTGAGTTT180.1525682319037125No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT180.1525682319037125Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGAATGAAGTAGACATGGGAGACTTTTTATTT170.1440922190201729No Hit
GCAAAAGTTCTCAGGGATGCTTCAGCAACTCTGCGTTGATACCACTGCTT160.13561620613663333Clontech SMART CDS Primer II A (95% over 24bp)
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTCACAGA150.12714019325309373No Hit
GGTGTTTGGGATGGAATGGTGGAGCGCTTGTTGTAGTGCGCCAGGCGGGA150.12714019325309373No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA150.12714019325309373No Hit
GGTGTTTGGGATGGAATGGTGCGGCCACTGATAGGATCACAGAGACCAAG140.11866418036955416No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG140.11866418036955416No Hit
GTGCTGTCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAT130.11018816748601457No Hit
GCAAAAGTTCTCAGGGATGTTTCAGCAACTCTGCGTTGATACCACTGCTT130.11018816748601457Clontech SMART CDS Primer II A (95% over 24bp)
CCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA120.10171215460247499No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)