FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5704
Total Bases821.9 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT148826.08695652173913No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA106818.723702664796633No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT80314.077840112201963No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA5579.76507713884993No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGT2724.768583450210379No Hit
CAACCAAAAGAGAAGGAGGCACGCCTCAAGGAATAGGTTCTCCTAGTGTC490.8590462833099579No Hit
GCATCAATCATCACAGGGTTGGGTAGGAAGGAACTTGGAACAGAACAGAA360.6311360448807855No Hit
CAACCAAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGT350.6136044880785414No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC310.5434782608695652No Hit
CAACCAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG190.3330995792426367No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA190.3330995792426367Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAAGGAGGCACTGAAGGGAGAAGGAAAGATAGTCTTGAA190.3330995792426367No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCATGT170.2980364656381487No Hit
CAACCAAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTCAGTGTA150.26297335203366057No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC140.24544179523141654No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG120.21037868162692847No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG110.1928471248246844No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT110.1928471248246844No Hit
CAACCAAAAGAGAAGGGGGCACCACAGGTTTCTGGATCCAGCCCCTGACA110.1928471248246844No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT100.1753155680224404No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC100.1753155680224404No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA100.1753155680224404No Hit
CAACCAAAAGAGAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG100.1753155680224404No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT100.1753155680224404No Hit
CAACAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG90.15778401122019636No Hit
TCCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT90.15778401122019636No Hit
GCATCAATCATCACAGGGTTGATTCGGCTGATCTGGCTGGCTAGGCGGGT80.1402524544179523No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT80.1402524544179523No Hit
GCATCAATCATCACAGGGTTGGAAGCAGTGGTATCAACGCAGAGTACATG80.1402524544179523Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT80.1402524544179523No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC80.1402524544179523Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG80.1402524544179523No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT80.1402524544179523No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCCTCACTTACTCT70.12272089761570827No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA70.12272089761570827No Hit
CAACCAAAAGAGAAGGAGGCACTAGGGATCGTCCGCAGGATTGGGACTGA70.12272089761570827No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT70.12272089761570827No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATAAAAGA70.12272089761570827No Hit
GCATCAATCATCACGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG70.12272089761570827No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG60.10518934081346423No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCATGTT60.10518934081346423No Hit
CAACCAAAAGAGAAGGAGGCACTGGTGACAGCGAAGAAGTGCAAGTGGGT60.10518934081346423No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT60.10518934081346423No Hit
CAACCAAAAGAGAAGGAGGCACTGGCAGAGGTGCCATAGAAGCAGTGAAC60.10518934081346423No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGGCAGCACCATCAAAGA60.10518934081346423No Hit
CAACCAAAAGAGAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGTA60.10518934081346423No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT60.10518934081346423No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT60.10518934081346423No Hit
AACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG60.10518934081346423No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCATCTT60.10518934081346423No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)