FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66912
Total Bases9.7 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGTCTCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCAT2579538.55063366810138No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA2103031.4293400286944No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA678010.13271162123386No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC8011.1970946915351506No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTCACAGAGA5510.823469631755141No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATCACAGAGACCAAGTCTCTG5230.78162362505978No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT4540.6785031085604974No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATCACAGAGACCAAGTCTCTG3710.5544595887135342No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA3580.5350310856049737No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT2950.44087757054041127No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG2290.34224055475848875No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG2150.32131755141080826No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG2080.31085604973696795No Hit
GTGCTGTCTCTCTTTATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT1870.27947154471544716No Hit
GTGCTGTCTCTCTTTGATGGATCGCTACAGATCCAGGAGTGGGGCCCATT1840.274988043998087No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA1460.2181970349115256No Hit
GTGCTGTCTCTCTTTGATGGAATCACTACAGATCCAGGAGTGGGGCCCAT1460.2181970349115256No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1400.20923003347680535No Hit
GTGCTGTCTCTTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT1190.17784552845528454No Hit
TGCTGTCTCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT1140.17037302725968437No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATCACAGA1120.1673840267814443No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA1080.16140602582496413No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATCACAGAGACCAAGTCTCTG1030.15393352462936394No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC1020.1524390243902439No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA990.14795552367288378No Hit
GTGCTGTCTCCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT980.14646102343376374No Hit
GTGCTGTCTCTCTTTGTGGAATCGCTACAGATCCAGGAGTGGGGCCCATT950.14197752271640363No Hit
GTGCTGTCTCTCTTTGATGAATCGCTACAGATCCAGGAGTGGGGCCCATT920.1374940219990435No Hit
GTGCCTCCTTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTA900.13450502152080346No Hit
GTGCTGTCTTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCATT810.12105451936872309No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG770.11507651841224295No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA690.10312051649928264No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)