FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2196
Total Bases318.1 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA86439.34426229508197No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC1768.014571948998178No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTCACAGAGA1757.969034608378871No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT944.280510018214937No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA853.8706739526411655No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCACTGCTTCC522.3679417122040074Clontech Universal Primer Mix Long (96% over 25bp)
GTGCCTCCTTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTA291.3205828779599271No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG130.5919854280510018No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG120.546448087431694No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCACCCCATGT120.546448087431694No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATCACAGAGACCAAGTCTCTG120.546448087431694No Hit
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTCACAGA110.5009107468123861No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATCACAGAGACCAAGTCTCTG110.5009107468123861No Hit
GTGCTGTCTCTCTTTGATGGACCCTGTTGCTTCCTCTCTCTAATGTTTCC110.5009107468123861No Hit
GTGCTGTCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAC110.5009107468123861No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG110.5009107468123861No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCAGTCTCTC90.4098360655737705No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCCCATGTACT90.4098360655737705No Hit
GTGCTGTCTCTCTTTGATGGAGGATAAAGCAGTTTTTTTTTTATTTTTAA80.36429872495446264No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCACTGTCCCA80.36429872495446264Clontech SMART CDS Primer II A (95% over 21bp)
GTGCCTCCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAC70.31876138433515483No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA70.31876138433515483No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG60.273224043715847No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCGGTCTCTC60.273224043715847No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC60.273224043715847No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCATCCCATGT50.22768670309653918No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCGGCA50.22768670309653918No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTGGTTGTCACAGAGAC50.22768670309653918No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATCACAGA50.22768670309653918No Hit
GTGCTGTCTCTCTTTGATGGACCTGAGCAACTCTGCGTTGATACCACTGC40.18214936247723132Clontech SMART CDS Primer II A (95% over 22bp)
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC40.18214936247723132No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG40.18214936247723132No Hit
GCAAAAGTTCTCAGGGATGTGCAACTCTGCGTTGATACCACTGCTTCCCA40.18214936247723132Clontech Universal Primer Mix Long (96% over 26bp)
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG40.18214936247723132No Hit
GTGCTGTCTCTCTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTACA40.18214936247723132No Hit
GTGCTGTCTCTCTTTGATGGAAATACGGCAGCGCATCCACCACAACCTCT40.18214936247723132No Hit
GTGCTGTCTCTCTTTGATGGAATGCTCTTGCACCCTTGTCAGAGACCACC30.1366120218579235No Hit
GTGCTGTCTCTCTTTGATGGATCCACAAAATCCCAAGTAGCAGGATTGCT30.1366120218579235No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCACTGTTCCC30.1366120218579235Clontech Universal Primer Mix Long (95% over 22bp)
GTGCTGTCTCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCAT30.1366120218579235No Hit
GTGCTGTCTCTCTTTGATGGTGGTGCTGTCTCTCTTTGATGGTGGTGCTG30.1366120218579235No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGATGTCTCTC30.1366120218579235No Hit
GGTGTTTGGGATGGAATGGTGGTCCACGCAGGTGGTTCGCAGAAGCAGCA30.1366120218579235No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAGGCAGCA30.1366120218579235No Hit
GGTGTTTGGGATGGAATGGTGATCTACGCAGGTGGTTCGCAGAAGCAGCA30.1366120218579235No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCGGGTGGTTCGCAGAAGCAGCA30.1366120218579235No Hit
GTGCTGTCTCTCTTTGATGGAATCCAGTCAAGTGGATGGCAAAAATCACT30.1366120218579235No Hit
GCAAAAGTTCTCAGGGATGTGAGCAACTCTGCGTTGATACCACTCTCCCA30.1366120218579235No Hit
GTGCTGTCTCTCTTTGATGGAATCCATGCCTGAATCTGCGGTATACAATT30.1366120218579235No Hit
GGTGTTTGGGATGGAATGGTGATCCGCGCAGGTGGTTCGCAGAAGCAGCA30.1366120218579235No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGACTCTC30.1366120218579235No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT30.1366120218579235No Hit
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTCACAGAG30.1366120218579235No Hit
GCAAAAGTTCTCAGGGATGTCCAACCCTGTGATGATTGATGCTCACAGAG30.1366120218579235No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCGGGCTCTC30.1366120218579235No Hit
GTGCTGTCTCTCTTTGATGGAATCTATCACTTACCAACCCTGTGATGATT30.1366120218579235No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGGACCCCCAT30.1366120218579235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)