FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2196
Total Bases318.2 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT86039.162112932604735No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA31214.207650273224044No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGT1737.877959927140255No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA863.9162112932604733No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA723.278688524590164Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC371.6848816029143898Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGT301.366120218579235No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA170.7741347905282332Clontech Universal Primer Mix Long (96% over 26bp)
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC150.6830601092896175Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCATTTACTCT150.6830601092896175No Hit
CAACCAAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTCAGTGTA130.5919854280510018No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC120.546448087431694No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA120.546448087431694Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC110.5009107468123861No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCATGTT110.5009107468123861No Hit
CAACCAAAAGAGAAGGAGGCACAGAACAGGGGCTCCCCTCATGGCTGCAG100.45537340619307837No Hit
CAACCAAAAGAGAAGGAGGCACTGCTACAACCATGGTGTCTAAAGATATA100.45537340619307837No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA90.4098360655737705No Hit
GCATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG70.31876138433515483Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA70.31876138433515483No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC70.31876138433515483Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT70.31876138433515483No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT70.31876138433515483No Hit
CAACCAAAAGAGAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGTA60.273224043715847No Hit
TCCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT60.273224043715847No Hit
GCATCAATCATCACAGGGTTGGTGGTACCCTCGAGCACACCAGACTTGCA60.273224043715847No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT50.22768670309653918No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCATGTT50.22768670309653918No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGTA50.22768670309653918No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT50.22768670309653918No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC50.22768670309653918No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATGTTG40.18214936247723132No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT40.18214936247723132No Hit
GCATCAATCATCACAGGGTTGGCTTGAAAAACGGGCTTATAGGAGGTTTA40.18214936247723132No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT40.18214936247723132No Hit
GCATCAATCATCACAGGGTTGGAAGCAGTGGTATCAACGCAGAGTACATG40.18214936247723132Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACAGTCCAAAGAGATCGGGGAAGATTCCTC40.18214936247723132No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCAACAAAGA40.18214936247723132No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG40.18214936247723132No Hit
GCATCAATCATCACAGGGTTGGTAAGTGATAGATTCCATCAAAGAGAGAC40.18214936247723132No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT40.18214936247723132No Hit
GCATCAATCATCACAGGGTTGGTGGCTGGAGAGGTTGTGGTGGATGCGCT40.18214936247723132No Hit
CAACCAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTCAGTGTAG40.18214936247723132No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG40.18214936247723132No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATAAAAGA30.1366120218579235No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT30.1366120218579235No Hit
CAACCAAAAGGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG30.1366120218579235No Hit
CAACCAAAAGAGAAGGAGGCACCTAAAAATTGTATACCGCAGATTCAGGC30.1366120218579235No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT30.1366120218579235No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT30.1366120218579235No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACGTACTCT30.1366120218579235No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCC30.1366120218579235No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT30.1366120218579235No Hit
GCATCAATCATCACAGGGTTGGACATCCCTGAGAACTTTTGCCCATGTTG30.1366120218579235No Hit
CAACAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGTA30.1366120218579235No Hit
AACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG30.1366120218579235No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT30.1366120218579235No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA30.1366120218579235No Hit
GCATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG30.1366120218579235Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)