FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3286
Total Bases476.1 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA106632.44065733414486No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC43213.146682897139378No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTCACAGAGA33310.13390139987827No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA3039.220937309799147No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT1594.838709677419355No Hit
GTGCCTCCTTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTA381.1564211807668898No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC280.852099817407182No Hit
GTGCTGTCTCTCTTTGATGGAGGACATGGGAGACTTTTCATTTTGTTCTG280.852099817407182No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATCACAGAGACCAAGTCTCTG230.699939135727328No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATCACAGAGACCAAGTCTCTG210.6390748630553865No Hit
GTGCTGTCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAT150.4564820450395618No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCGGTCTCTC140.426049908703591No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTCACAG130.39561777236762025No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG120.36518563603164944No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG110.33475349969567864No Hit
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTCACAGA100.30432136335970783No Hit
GTGCCTCCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAT100.30432136335970783No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA80.24345709068776628No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCCTCC80.24345709068776628No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG80.24345709068776628No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCAGTCTCTC70.2130249543517955No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC70.2130249543517955No Hit
GTGCTGTCTCTCTTTGATGGACTTCAGACCATTCTCCTCCCGCATGGCCT70.2130249543517955No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG70.2130249543517955No Hit
GTGCTGTCTCTCTTTGATGGAAGACATGGGAGACTTTTCATTTTGTTCTG60.18259281801582472No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGATGTCTCTC60.18259281801582472No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCAGTC60.18259281801582472No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGACTCTC60.18259281801582472No Hit
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTCACAGAG60.18259281801582472No Hit
GGTGTTTGGGATGGAATGGTGATCCGCGCAGGTGGTTCGCAGAAGCAGCA50.15216068167985392No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC50.15216068167985392No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTCTCTTTTGGTTGTCACAGAGAC40.12172854534388314No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA40.12172854534388314No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCCTCCTTCTCTTTTGGTTGTCACAG40.12172854534388314No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTGGTTGTCACAGAGAC40.12172854534388314No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG40.12172854534388314No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTTCACAGAGAC40.12172854534388314No Hit
GTGCTGTCTCTCTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTATT40.12172854534388314No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTCACAGAGACCAAGTCTCT40.12172854534388314No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT40.12172854534388314Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG40.12172854534388314No Hit
GTGCTGTCTCTCTTTGATGGAATCGAAAGGCGGGAAAGTGGGGAAAAGAT40.12172854534388314No Hit
GGTGTTTGGGATGGAATGTTGATCCACGCAGGTGGTTCGCAGAAGCAGCA40.12172854534388314No Hit
GGGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG40.12172854534388314No Hit
CTCCAAGAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA40.12172854534388314No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCCT40.12172854534388314No Hit
GTGCTGTCTCTCTTTGATGGAGTGCCTCCTTCTCTTTTGGTTGTCACAGA40.12172854534388314No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATCACAGA40.12172854534388314No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)