FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3414
Total Bases494.6 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA143642.06209724663152No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTCACAGAGA1534.481546572934974No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC1524.452255418863503No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC1484.335090802577621No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT581.698886936145284No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATCACAGAGACCAAGTCTCTG521.5231400117164615No Hit
GTGCTGTCTCTCTTTGATGGATGACCAAAGGAGATCCCCTTTAAGGAGGT491.4352665495020505No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATCACAGAGACCAAGTCTCTG411.200937316930287No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT401.1716461628588166Clontech Universal Primer Mix Long (96% over 26bp)
GTGCCTCCTTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTA330.9666080843585236No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT320.9373169302870533Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT300.8787346221441126No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA300.8787346221441126No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA260.7615700058582308No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA240.7029876977152899No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA220.6444053895723492No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC220.6444053895723492No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC190.5565319273579379Clontech SMART CDS Primer II A (95% over 24bp)
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA160.46865846514352666No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATCACAGAGACCAAGTCTCTG150.4393673110720563No Hit
GTGCTGTCTCTCTTTGATGGAATCAGTGTAAAGTGAGTCCTCTCAAACCT150.4393673110720563No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA130.3807850029291154No Hit
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTCACAGAG130.3807850029291154No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC130.3807850029291154No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG120.35149384885764495No Hit
GTGCTGTCTCTCTTTGATGGAATCAGTGGTATCAACGCAGAGTACATGGG110.3222026947861746Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAG110.3222026947861746No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATCACAGA110.3222026947861746No Hit
CTCCAAGTAAGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA100.29291154071470415No Hit
GTGCTGTCTCTCTTTGATGGACCACCTCTTTGGGGCCCTGGTCCTCCTGC90.26362038664323373No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTCACAGAGACCAAGTCTCT80.23432923257176333No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCATGTA80.23432923257176333Clontech SMART CDS Primer II A (96% over 26bp)
CTCCAAGTAACGGTGCGAGGGTAAAGGGCTCCTGGTGCTGAAGGACTTCG70.2050380785002929No Hit
GTGCTGTCTCTCTTTGATGGACAAGTCACCCAAGCAGGTTGACATCAGGT70.2050380785002929No Hit
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTCACAGA60.17574692442882248No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG60.17574692442882248No Hit
GCAAAAGTTCTCAGGGATGTGCAACTCTGCGTTGATACCACTGCTTCCCA60.17574692442882248Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTCACAGAGACCA50.14645577035735208No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTCACAGAGACCAAG50.14645577035735208No Hit
GTGCCTCCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAG50.14645577035735208No Hit
GCAAAAGTTCTCAGGGATGAGCAACTCTGCGTTGATACCACTGCTTCCCA50.14645577035735208Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAAGTAACGGTGCTGTCTACTGGCTGGTGCAGGGCTCCTGGTGCTGA50.14645577035735208No Hit
GTGCTGTCTCTCTTTGATGGAGGTCATCCCCAAGCAGCTTAGTGAGGACC50.14645577035735208No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTCACAGAGACCA50.14645577035735208No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCCTCCTT50.14645577035735208No Hit
GCAAAAGTTCTCAGGGATGTGCGTTGATACCACTGCTTCCCATGTACTCT50.14645577035735208Clontech Universal Primer Mix Long (95% over 21bp)
GTGCTGTCTCTCTTTGATGCCACTGCTTCCCATGTACTCTGCGTTGATAC50.14645577035735208No Hit
GTGCTGTCTCTCTTTGATGGAGGTCATCCCCAAGCAGCTGCGTTGATACC40.11716461628588166No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT40.11716461628588166Clontech SMART CDS Primer II A (96% over 25bp)
GTGCTGTCTCTCTTTGATGGAATCCATGATCGCATCAAACACCTACATTC40.11716461628588166No Hit
CTCCAAGTAACGGTGCGAGGGTAAAGGGCTCCTGGTGCTGAAGGACTTGG40.11716461628588166No Hit
GTGCTGTCTCTCTTTGATGGACACTGACGACATGGTTCTACAACATGGGT40.11716461628588166No Hit
GTGCTGTCTCTCTTTGATGGACAAGTCACCCAAGCAGGTTTACATCAGGT40.11716461628588166No Hit
GTGCTGTCTCTCTTTGATGGAAGCAGTGGTATCAACGCAGAGTACATGGG40.11716461628588166Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGCTGTCTCTCTTTGATGGACTCTGCGTTGATACCACTGCTTCCCATGT40.11716461628588166Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAAGTAACGGTGCTGTCTGCTGGTTGGTGCAGGGCTCCTGGTGCTGA40.11716461628588166No Hit
GGTGTTTGGGATGGAATGGTATCAACGCAGAGTACATGGGAAGCAGTGGT40.11716461628588166Clontech SMARTer II A Oligonucleotide (95% over 21bp)
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGTAGGGCTCCTGGTGCTGA40.11716461628588166No Hit
CCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA40.11716461628588166No Hit
GCAAAAGTTCTCAGGGATGTGAGGCCCACCCATGTACTCTGCGTTGATAC40.11716461628588166No Hit
GCAAAAGTTCTCAGGGATGTGGAAGAGAAAGAATACCCATGTACTCTGCG40.11716461628588166No Hit
CTCCAAGTAACGGTGCTGCCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA40.11716461628588166No Hit
GTGCTGTCTCTCTTTGATGGACCTAGGCTTGACCCATGTACTCTGCGTTG40.11716461628588166No Hit
GCAAAAGTTCTCAGGGATGTGTCATCCCCAAGCAGCTTAGTGAGGACCAC40.11716461628588166No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)