FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4847
Total Bases702.3 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA109122.50876831029503No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA72915.040231070765422No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC2785.735506498865277No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTCACAGAGA2755.673612543841552No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA2575.302248813699196No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT1372.826490612750155No Hit
GTGCTGTCTCTCTTTGATGGAATCGAAAGGCGGGAAAGGTGGGGAAAAGA1172.4138642459253146No Hit
GCAAAAGTTCTCAGGGATGTAGCAACTCTGCGTTGATACCACTGCTTCCC621.2791417371570044Clontech Universal Primer Mix Long (96% over 26bp)
GCAAAAGTTCTCAGGGATGTCGCAACTCTGCGTTGATACCACTGCTTCCC591.2172477821332783Clontech Universal Primer Mix Long (96% over 26bp)
GTGCCTCCTTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTA501.0315659170621003No Hit
GCAAAAGTTCTCAGGGATGTAGCAACTCTGCGTTGATACCACCCCATGTA430.8871466886734063No Hit
GCAAAAGTTCTCAGGGATGTCGCAACTCTGCGTTGATACCACCCCATGTA410.8458840519909222No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATCACAGAGACCAAGTCTCTG400.8252527336496802No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATCACAGAGACCAAGTCTCTG370.7633587786259541No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA250.5157829585310502No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATCACAGA240.4951516401898081No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAAGATGTAGCAAC240.4951516401898081No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGGGGTGTTTGGGATG230.4745203218485661No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC220.45388900350732414No Hit
GTGCTGTCTCTCTTTGATGGACAAGAGCAGAAGGCGAAGGGGGAGCCTCT210.4332576851660821No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC200.4126263668248401No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA200.4126263668248401No Hit
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTCACAGA190.3919950484835981No Hit
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTCACAGAG190.3919950484835981No Hit
GCAAAAGTTCTCAGGGATGTCGCAACTCTGCGTTGATACCCCATGTACTC180.37136373014235613No Hit
GCAAAAGTTCTCAGGGATGTCGCAACCCTGTGATGATTGATGCTCACAGA160.3301010934598721No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATCACAGAGACCAAGTCTCTG160.3301010934598721No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA160.3301010934598721No Hit
GTGCTGTCTCTCTTTGATGGAGATTGTGAACTCTTCATTACAGAGTGCAT160.3301010934598721No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC160.3301010934598721No Hit
GCAAAAGTTCTCAGGGATGTAGCAACTCTGCGTTGATACCCCATGTACTC150.30946977511863005No Hit
GCAAAAGTTCTCAGGGATGTAGCAACTCTGCGTTGATACCACCCATGTAC130.2682071384361461No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA120.24757582009490406No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCCTCC120.24757582009490406No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC120.24757582009490406No Hit
GTGCTGTCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAT110.22694450175366207No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA110.22694450175366207No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTCACAGAGACCAAG100.20631318341242005No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCCTCCTTCTCTTTTGGTTGTCACAG100.20631318341242005No Hit
GCAAAAGTTCTCAGGGATGTCGCAACTCTGCGTTGATACCACCCATGTAC100.20631318341242005No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG90.18568186507117806No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG90.18568186507117806No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCAGTCTCTC90.18568186507117806No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGACTCTC90.18568186507117806No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCCTCCTT90.18568186507117806No Hit
GGTGTTTGGGATGGAATGGTATCAACGCAGGTGGTTCGCAGAAGCAGCAG80.16505054672993605No Hit
GTGCTGTCTCTCTTTGATGGAATCAGTGGTATCAACGCAGAGTACATGGG80.16505054672993605Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGAATCTCTCAGCCTCCTTCTCTTTTGGTTGT80.16505054672993605No Hit
GTGCTGTCTCTCTTTGATGGAGCAACTCTGCGTTGATACCACTGCTTCCC80.16505054672993605Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG70.14441922838869403No Hit
CTCCAAGTAACGGTGCTGTCTACTGGCTGGTGCAGGGCTCCTGGTGCTGA70.14441922838869403No Hit
GTGCTGTCTCTCTTTGATGGACCATTGATTAGGTTACTAAGTGTGCCTCC70.14441922838869403No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA70.14441922838869403No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG60.12378791004745203No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT60.12378791004745203No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGTTGTCACAGAGAC60.12378791004745203No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA60.12378791004745203No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTCACAGAGACCAAGTCTCT60.12378791004745203No Hit
GTGCTGTCTCTCTTTGATGGACACGGAAATGTTGAATACTCATCTTGTGC60.12378791004745203No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCTCACAGAGACCA60.12378791004745203No Hit
GCAAAAGTTCTCAGGGATGTAGCAACTCTGCGTTGATACCACTGCTTCCA60.12378791004745203Clontech Universal Primer Mix Long (96% over 25bp)
GTGCTGTCTCTCTTTGATGCCACTGCTTCCCATGTACTCTGCGTTGATAC60.12378791004745203No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTCAAGATGTAGCAAC50.10315659170621003No Hit
GCAAAAGTTCTCAGGGATGTCGCAACTCTGCGTTGATACCACTGCTTCCA50.10315659170621003Clontech Universal Primer Mix Long (96% over 25bp)
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCGGTCTCTC50.10315659170621003No Hit
GTGCCTCCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAT50.10315659170621003No Hit
CCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA50.10315659170621003No Hit
GTGCTGTCTCTCTTTGATGGAATCTAGCCATGGGTCTGCTCTAGCCTGGG50.10315659170621003No Hit
GTGCTGTCTCTCTTTGATGGAATCCTTCATTCTTAACGATTGATATAGTT50.10315659170621003No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCGGGGCTCCTGGTGCTGA50.10315659170621003No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCGGTC50.10315659170621003No Hit
GCAAAAGTTCTCAGGGATGTAGCAACTCTGCGTTGATACCACTGCCCATG50.10315659170621003Clontech SMART CDS Primer II A (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)