FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4847
Total Bases701.5 kbp
Sequences flagged as poor quality0
Sequence length136-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA105321.72477821332783No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT71914.833917887353001No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA4749.77924489374871No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGT2715.591087270476583No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT2535.219723540334227No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1783.6723746647410773Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC1252.5789147926552505Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGGGTTGGGGGCAAGGTCACAGATCAACAGGATCCC1172.4138642459253146No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA621.2791417371570044Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGT531.0934598720858262No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC410.8458840519909222No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC390.8046214153084381Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC360.7427274602847123No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA310.6395708685785022No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA260.5364142768722922No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA260.5364142768722922No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA250.5157829585310502Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA250.5157829585310502No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCATGTT240.4951516401898081No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA220.45388900350732414No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCATGTT210.4332576851660821No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA200.4126263668248401No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATGTTG190.3919950484835981No Hit
GCATCAATCATCACAGGGTTGGTGCTGGGTGAGGGCCTGCCCCACACAGA190.3919950484835981No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC180.37136373014235613Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG180.37136373014235613Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC160.3301010934598721No Hit
GCATCAATCATCACAGGGTTGCGACATCCCTGAGAACTTTTGCCCATGTT150.30946977511863005No Hit
CAACCAAAAGAGAAGGAGGCACAGTTGGATGATAGGACAAAAATCACTGA130.2682071384361461No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA120.24757582009490406No Hit
CAACCAAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTCAGTGTA110.22694450175366207No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA110.22694450175366207No Hit
GCATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG100.20631318341242005Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCCATGT100.20631318341242005No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGAAC100.20631318341242005No Hit
CAACCAAAAGAGAAGGAGGCTGAGAGATTCCATCAAAGAGAGACAGCACC90.18568186507117806No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA90.18568186507117806No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT90.18568186507117806No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCAACAAAGA80.16505054672993605No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT80.16505054672993605No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA80.16505054672993605No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT70.14441922838869403No Hit
GCATCAATCATCACAGGGTTGGAAGCAGTGGTATCAACGCAGAGTACATG70.14441922838869403Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA70.14441922838869403No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT70.14441922838869403No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG70.14441922838869403No Hit
CAACCAAAAGAGAAGGAGGCACAGATCCAACTATATAAATCATAACATTA70.14441922838869403No Hit
CAACCAAAAGAGAAGGAGGCACACTTAGTAACCTAATCAATGGTCCATCA70.14441922838869403No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCATGTTGTAGA60.12378791004745203No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGT60.12378791004745203No Hit
GCATCAATCATCACAGGGTTGGGGCCACTTTCTCATCGGTTGGGTTAGGC60.12378791004745203No Hit
CAACAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGTA60.12378791004745203No Hit
CAACCAAAAGAGAAGGAGGCACAAGATGAGTATTCAACATTTCCGTGTCC60.12378791004745203No Hit
CAACCAAAAGAGAAGGGGGCACCATCAAAGAGAGACAGCACCATCAAAGA60.12378791004745203No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCAAAGA50.10315659170621003No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT50.10315659170621003No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAGGGAGGCA50.10315659170621003No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCG50.10315659170621003No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)