FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1312
Total Bases189.9 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT32424.695121951219512No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA18013.719512195121952No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA1229.298780487804878No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGT826.25No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA715.411585365853658Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT634.801829268292683No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA191.4481707317073171Clontech Universal Primer Mix Long (96% over 26bp)
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC181.3719512195121952No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA161.2195121951219512No Hit
CAACCAAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGT161.2195121951219512No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC151.1432926829268293Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC141.0670731707317074No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA120.9146341463414633No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC100.7621951219512195Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCATGTT90.6859756097560976No Hit
CAACCAAAAGAGAAGGAGGCACTCATCGTCCCTCACAGCAGCCGCCGCCG80.6097560975609756No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATGTTG70.5335365853658537No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC70.5335365853658537Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA60.45731707317073167No Hit
GCATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG60.45731707317073167Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCATGTT50.38109756097560976No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA50.38109756097560976Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT50.38109756097560976No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC50.38109756097560976No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA50.38109756097560976No Hit
GCATCAATCATCACAGGGTTGGCCGACCTGATGTCAACCTGCTTGGGTGA40.3048780487804878No Hit
GCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAAG40.3048780487804878No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA40.3048780487804878No Hit
GCATCAATCATCACAGGGTTGGAAGCAGTGGTATCAACGCAGAGTACATG30.22865853658536583Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCAACAAAGA30.22865853658536583No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT30.22865853658536583No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA30.22865853658536583No Hit
GCATCAATCATCACAGGGTTGGACTCGGGAAATCGCGAGATGTCCCTCTT20.1524390243902439No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATAAAAGA20.1524390243902439No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACTCCATCA20.1524390243902439No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA20.1524390243902439No Hit
GATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG20.1524390243902439No Hit
CAACCAAAAGAGAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGTA20.1524390243902439No Hit
CAACCAAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTCAGTGTA20.1524390243902439No Hit
GGGCAAAGGGTGAAGAGAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA20.1524390243902439No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATCAAAG20.1524390243902439No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCCATGT20.1524390243902439No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGAAC20.1524390243902439No Hit
GCATCAATCATCACAGGGTTGCGGACATCCCTGAGAACTTTTGCCCATGT20.1524390243902439No Hit
CACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG20.1524390243902439No Hit
GGGCAAAGGGTGAAGGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA20.1524390243902439No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAGTGAAAGGAGGCA20.1524390243902439No Hit
GCATCAATCATCACAGGGTTGCCGACCTGATGTCAACCTGCTTGGGTGAC20.1524390243902439No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGAAAGCACCCATGTTGT20.1524390243902439No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCGCCCATGTTGT20.1524390243902439No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG20.1524390243902439No Hit
AACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG20.1524390243902439No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.1524390243902439No Hit
GCATCAATCATCACAGGGTTGGCCGACCTGATGTGAACCTGCTTGGGTGA20.1524390243902439No Hit
CAACCAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGTA20.1524390243902439No Hit
GCATCAATCATCACAGGGTACATGGGAATCAGTGGTATCAACGCAGAGTA20.1524390243902439Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TCCTATCAGTGGCCGCAAAGCAGTGGTATCAACGCAGAGTACATGGGAAG20.1524390243902439Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGGGGCACCATCAAAGAGAGACAGCACCATCAAAGA20.1524390243902439No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.1524390243902439No Hit
CAACCAAAAGAGAAGGAGGCACGATTCCATCAAAGAGAGACAGCACCCAT20.1524390243902439No Hit
CAACCAAAAGAGAAGGAGGCACAAGATGAGTATTCAACATTTCCGTGTCC20.1524390243902439No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG20.1524390243902439No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)