FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1350
Total Bases194.9 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC54

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA23217.185185185185183No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT14610.814814814814815No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA1329.777777777777779No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGT1299.555555555555555No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT1118.222222222222223No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA987.2592592592592595Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA362.666666666666667No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA241.7777777777777777Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGT211.5555555555555556No Hit
GCATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG130.962962962962963Clontech Universal Primer Mix Long (96% over 26bp)
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC90.6666666666666667Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCAGTGCTAGGAGAAAAGAAAGCCTCTCT80.5925925925925926No Hit
GCATCAATCATCACAGGGTTGGAAGCAGTGGTATCAACGCAGAGTACATG80.5925925925925926Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC70.5185185185185185No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC60.4444444444444444No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCATGTT60.4444444444444444No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA60.4444444444444444No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA60.4444444444444444No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC60.4444444444444444Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACGGAGACAGAAAGTGGGAACATTAAGCAG60.4444444444444444No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATAAAAGA50.3703703703703704No Hit
CAACCAAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTCAGTGTA50.3703703703703704No Hit
CAACCAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG40.2962962962962963No Hit
CAACCAAAAGAGAAGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG40.2962962962962963No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATGTTG40.2962962962962963No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA40.2962962962962963Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGGGGCACCATCAAAGAGAGACAGCACCATCAAAGA40.2962962962962963No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCCATGT30.2222222222222222No Hit
CACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG30.2222222222222222No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC30.2222222222222222Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCATGT30.2222222222222222No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG30.2222222222222222No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCAAAGA30.2222222222222222No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGCCCCTCTT30.2222222222222222No Hit
GCATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG30.2222222222222222Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG20.14814814814814814No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGTCCTGGGCTTCACTTACTCT20.14814814814814814No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG20.14814814814814814No Hit
GCATCAATCATCACAGGGTACATGGGCATGGAAGCAGTGGTATCAACGCA20.14814814814814814Clontech Universal Primer Mix Long (95% over 21bp)
CAACCAAAAGAGAAGGAGGCCAAACCACCCCTGGCACCATCAGGAGGCAC20.14814814814814814No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT20.14814814814814814No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG20.14814814814814814No Hit
CAACCAAAAGAGAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGTA20.14814814814814814No Hit
CAACCAAAAGAGAAGGAGGCACTGAGGGGCCAGAGCAGCCCCCACCACCT20.14814814814814814No Hit
CAACCAAAAGAGAAGGAGGCACCCTCCATCAAAGAGAGACAGCACCCATG20.14814814814814814No Hit
GCATCAATCACCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA20.14814814814814814Clontech Universal Primer Mix Long (96% over 26bp)
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGACGTCCCTCTT20.14814814814814814No Hit
CAACCAAAAGAGAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG20.14814814814814814No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAATGA20.14814814814814814No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCATGTT20.14814814814814814No Hit
CAACCAAAAGAGAAGGAGGCACTGTGGCCGAGAAGCTGGACTGGGCCCGC20.14814814814814814No Hit
GCATCAATCATCAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTC20.14814814814814814No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCATAGA20.14814814814814814No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG20.14814814814814814No Hit
GCATCAATCTTCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA20.14814814814814814Clontech Universal Primer Mix Long (96% over 26bp)
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG20.14814814814814814No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGTA20.14814814814814814No Hit
GCATCAATCTTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT20.14814814814814814No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC20.14814814814814814No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG20.14814814814814814No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)