FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3745
Total Bases542.6 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA82321.975967957276367No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA3018.037383177570094No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC1834.886515353805073No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTCACAGAGA1734.619492656875835No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC1694.512683578104139No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA1393.711615487316422No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCTCACAGAGACCAAG962.563417890520694No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATCACAGAGACCAAGTCTCTG952.53671562082777No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATCACAGAGACCAAGTCTCTG681.8157543391188251No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT661.7623497997329773No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT491.3084112149532712No Hit
GTGCTGTCTCTCTTTGATGGAATCAGTGTAAAGTGAGTCCTCTCAAACCT461.2283044058744994No Hit
GTGCTGTCTCTCTTTGATGGAATCTGGTTTGTGGCCTCACTTGCTCCTCC431.1481975967957276No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT370.9879839786381843Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCCTCCTTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTA360.9612817089452604No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT340.9078771695594126Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC260.6942590120160214No Hit
GTGCTGTCTCTCTTTGATGGAATCAAGAGAGGCTACAGGAATGGCGTTGA220.5874499332443258No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATCACAGA220.5874499332443258No Hit
CTCCAAGTAACGGTGCTTTCTCTTCACCCTTTGCCCTCACAGAGACCAAG210.5607476635514018No Hit
GTGCTGTCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAT190.507343124165554No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA170.4539385847797063No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATCACAGAGACCAAGTCTCTG150.4005340453938585No Hit
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTCACAGAG150.4005340453938585No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA150.4005340453938585No Hit
GTGCTGTCTCTCTTTGATGGAATCAGTGGTATCAACGCAGAGTACATGGG140.3738317757009346Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC130.3471295060080107No Hit
GTGCTGTCTCTCTTTGATGGATATGAAGCACCGCCAGGTCCTTTGAGTTT120.32042723631508674No Hit
GTGCTGTCTCTCTTTGATGGACTAAAGTTCATAGGTCATCCAACTTATGC120.32042723631508674No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA100.26702269692923897No Hit
GTGCTGTCTCTCTTTGATGGAATCTCCATGAAAGCAAGAGACCCTACCTG100.26702269692923897No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTCTGCGTTGATACCA100.26702269692923897No Hit
CTCCAAGTAACGGTGCTGTCTCTCACCCTTTGCCCTCACAGAGACCAAGT90.2403204272363151No Hit
GTGCCTCCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAT90.2403204272363151No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA90.2403204272363151No Hit
GTGCTGTCTCTCTTTGATGGACAAAGCAAATGAGACATACAGGGTCAACA90.2403204272363151No Hit
GTGCTGTCTCTCTTTGATGCCACTGCTTCCCATGTACTCTGCGTTGATAC90.2403204272363151No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG80.2136181575433912No Hit
GTGCTGTCTCTCTTTGATGGAATCCGCTCTTGCTTCTCTTTCTTGGGTGT80.2136181575433912No Hit
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTCACAGA80.2136181575433912No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCTCACAGAGACCAAGTCTCT80.2136181575433912No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA80.2136181575433912No Hit
CTCCAAGTAAGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA70.1869158878504673No Hit
GCAAAAGTTCTCAGGGATGTGTCATCCCCAAGCAGCGGTCATCCCCAAGC70.1869158878504673No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGTCACAG70.1869158878504673No Hit
GTGCTGTCTCTCTTTGATGGAATCGTGCCTCCTTCTCTTTTGGTTGTCAC60.16021361815754337No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCGGTCTCTC60.16021361815754337No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA60.16021361815754337No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG60.16021361815754337No Hit
GTGCTGTCTCTCTTTGATGGAGCCTTGTGGAAACATCCCAGGCAGAGCTT60.16021361815754337No Hit
GGTGTTTGGGATGGAATGGTGGAGCGCTTGTTGTAGTGCGCCAGGCGGGA60.16021361815754337No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC60.16021361815754337Clontech SMART CDS Primer II A (95% over 24bp)
GTGCTGTCTCTCTTTGATGGAGGTGATTAGGGTGATTTTTGCCTGATATG60.16021361815754337No Hit
GTGCTGTCTCTCTTTGATGGAGAAACATTCTGATTTGAAGGCATTTTCCG60.16021361815754337No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA50.13351134846461948No Hit
GTGCTGTCTCTCTTTGATGGACCTCCCTTTACTATGATGTTGCTTCCTCT50.13351134846461948No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCTCACAGAGACCA50.13351134846461948No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGTTGGTGCAGGGCTCCTGGTGCTGA50.13351134846461948No Hit
GTGCTGTCTCTCTTTGATGGAGGTCATCCCCAAGCAGCTTAGTGAGGACC50.13351134846461948No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGGACCCATGT50.13351134846461948No Hit
CTCCAAGAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA50.13351134846461948No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCCTCCTT50.13351134846461948No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG40.1068090787716956No Hit
CTCCAAGTAACGGTGCGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA40.1068090787716956No Hit
GCAAAAGTTCTCAGGGATGTGTCATCCCCAAGCAGCTGCGTTGATACCAC40.1068090787716956No Hit
GTGCTGTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTATCG40.1068090787716956No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCCTCC40.1068090787716956No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG40.1068090787716956No Hit
GGTGTTTGGGATGGAATGGTGACCCACGCAGGTGGTTCGCAGAAGCAGCA40.1068090787716956No Hit
GTGCTGTCTCTCTTTGATGGAGGGTGTGACCCTTGAGGTCAGAAGAGGCC40.1068090787716956No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGATGCTGA40.1068090787716956No Hit
GTGCTGTCTCTCTTTGATGGAGGTCATCCCCAAGCAGCTGCGTTGATACC40.1068090787716956No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG40.1068090787716956No Hit
GCAAAAGTTCTCAGGGATGTGTCATCCCCAAGCAGCTGGTCATCCCCAAG40.1068090787716956No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCAGTCTCTC40.1068090787716956No Hit
CTCCAAGTAACGGTGCGAGGGTAAAGGGCTCCTGGTGCTGAAGGACTTGG40.1068090787716956No Hit
CTCCAAGTAACGGTGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGACTTGG40.1068090787716956No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC40.1068090787716956No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGTTGAAGGA40.1068090787716956No Hit
GTGCTGTCTCTCTTTGATGGACCTAGGCTTGACCCATGTACTCTGCGTTG40.1068090787716956No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)