FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3745
Total Bases541.9 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA81521.762349799732977No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT3098.251001335113486No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA2887.6902536715620835No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2055.473965287049399Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGT1694.512683578104139No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT1463.8985313751668893No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGAAC932.4833110814419226No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC922.456608811748999No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC832.2162883845126835Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC661.7623497997329773No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA581.548731642189586Clontech Universal Primer Mix Long (96% over 26bp)
GCATCAATCATCACAGGGTTGGGTAGGAAGGAACTTGGAACAGAACAGAA461.2283044058744994No Hit
CAACCAAAAGAGAAGGATAAACCAGTCCTTGAACCATAACAGCAGAGCTC441.1748998664886516No Hit
CAACCAAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGT350.9345794392523363No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC340.9078771695594126Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC240.6408544726301735No Hit
GGGCAAAGGGTGAAGAGAAAGCACCGTTACTTGGAGCCATGTTGTAGAAC220.5874499332443258No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCATGTT220.5874499332443258No Hit
CAACCAAAAGAGAAGGAGGCACAAACTGAAAACAGCCTTTAAAAATTTCA210.5607476635514018No Hit
CAACCAAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTCAGTGTA190.507343124165554No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA180.4806408544726302Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG160.4272363150867824Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATGTTG150.4005340453938585No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA150.4005340453938585No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA150.4005340453938585No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC150.4005340453938585No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA140.3738317757009346No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA140.3738317757009346No Hit
GCATCAATCATCACAGGGTTGGAAGCAGTGGTATCAACGCAGAGTACATG130.3471295060080107Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG120.32042723631508674Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA120.32042723631508674No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA110.2937249666221629No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA110.2937249666221629No Hit
CAACCAAAAGAGAAGGAGGCCAGGGCATAAGTTGGATGACCTATGAACTT100.26702269692923897No Hit
CAACCAAAAGAGAAGGGGGCACCATCAAAGAGAGACAGCACCATCAAAGA100.26702269692923897No Hit
GGGCAAAGGGTGAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGAACC90.2403204272363151No Hit
CAACCAAAAGAGAAGGAGGCAGGTAGGGTCTCTTGCTTTCATGGAGATTC90.2403204272363151No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA90.2403204272363151No Hit
TCCTATCAGTGGCCGCAAAGAGAGCTACTCCATCTACGTGTACAAGGTGC90.2403204272363151No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGT80.2136181575433912No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT80.2136181575433912No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCATGTT70.1869158878504673No Hit
CAACCAAAAGAGAAGGAGGGGGAAGGTTTGACAATGGCCTGAGGTGGAAG70.1869158878504673No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCATGT70.1869158878504673No Hit
CAACCAAAAGAGAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGTA70.1869158878504673No Hit
GCATCAATCATCACAGGGTTGGCAGCTGCACTAAAGCCTGGGGCAGCTCC70.1869158878504673No Hit
GCATCAATCATCACAGGGTTGATTCGGCTGATCTGGCTGGCTAGGCGGGT60.16021361815754337No Hit
GCATCAATCATCACAGGGTACATGGGAATCAGTGGTATCAACGCAGAGTA60.16021361815754337Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAACCAAAAGAGAAGGAGGCACGATTCCATCAAAGAGAGACAGCACCCAT60.16021361815754337No Hit
CAACCAAAAGAGAAGGAGGGGGAAGGTGCTACACACTTTTAAACAACCAA60.16021361815754337No Hit
CAACCAAAAGAGAAGGAGGCACACACACACAAGGGGCAGGAGTGGAGCAC50.13351134846461948No Hit
CAACCAAAAGAGAAGGAGGCGGAGGAAGAGGAGGAGAAGGTAGCTACAGC50.13351134846461948No Hit
CAACCAAAAGAGAAGGAGGCTGCTGAGATGGGAAAGGGCTCCTTCAAGTA50.13351134846461948No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCAAAGA50.13351134846461948No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGA50.13351134846461948No Hit
CAACCAAAAGAGAAGGAGGCACAGGTAGCTGCAGCAGATGAGAAGAAGTT50.13351134846461948No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA40.1068090787716956No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC40.1068090787716956Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCAACAAAGA40.1068090787716956No Hit
GGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA40.1068090787716956No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACAATCAAAGA40.1068090787716956No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG40.1068090787716956No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATAAAAGA40.1068090787716956No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGGA40.1068090787716956Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCATCAATCATCACAGGGTTGGTTCGGCTGATCTGGCTGGCTAGGCGGGT40.1068090787716956No Hit
CAACCAAAAGAGAAGGAGGCAAAGGTAGCTGCAGCAGATGAGAAGAAGTT40.1068090787716956No Hit
GCATCAATCAACACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA40.1068090787716956Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGACAGCACCCATGTTGTAGAACCATGTCGTCAGTGTAGA40.1068090787716956No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)