FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5013
Total Bases726.7 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT238647.59624975064831No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA79915.938559744663875No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGT3346.662677039696788No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA2575.126670656293637No Hit
CAACCAAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGT420.8378216636744463No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC410.8178735288250548No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC370.7380809894274886No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA270.5385996409335727Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTCAGTGTA190.37901456213844004No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCATGTT180.3590664272890485No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT180.3590664272890485No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT170.33911829243965685No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT160.3191701575902653No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG160.3191701575902653No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT160.3191701575902653No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT150.2992220227408737No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT150.2992220227408737No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT140.27927388789148216No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCAACAAAGA130.2593257530420906No Hit
TCCTATCAGGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT130.2593257530420906No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCATGT120.23937761819269898No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG120.23937761819269898No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC120.23937761819269898No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT110.21942948334330742No Hit
CAACCAAAAGAGAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGTA110.21942948334330742No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC110.21942948334330742No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC100.1994813484939158Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGGGTTGGAAGCAGTGGTATCAACGCAGAGTACATG90.17953321364452424Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT90.17953321364452424No Hit
CAACCAAAAGAGAAGGGGGCACCATCAAAGAGAGACAGCACCCATGTTGT80.15958507879513265No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT80.15958507879513265No Hit
CACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG80.15958507879513265No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA80.15958507879513265No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCCTCACTTACTCT70.13963694394574108No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA70.13963694394574108No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATAAAAGA70.13963694394574108No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT70.13963694394574108No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA70.13963694394574108No Hit
CAACCAAAAGAGAAGGGGGCACCATCAAAGAGAGACAGCACCATCAAAGA70.13963694394574108No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATGTTG60.11968880909634949No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGACCCTGGGCTTCACTTACTCT60.11968880909634949No Hit
GCATCAATCATCACAGGGTTGGGGACCCCCACCATACATGCTCTGAAAAT60.11968880909634949No Hit
CAACCAAAAGAGAAGGAGGCACAAGCACCAGGCAATGGTTTACCAGTGGT60.11968880909634949No Hit
CAACAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG60.11968880909634949No Hit
TCCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT60.11968880909634949No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGTA60.11968880909634949No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)