FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23372
Total Bases3.3 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA917839.26921102173541No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA428718.34246106452165No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA16176.918534999144275No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTCACAGAGA11695.001711449597809No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC10464.475440698271436No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT5402.3104569570426152No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG2411.03114838268013No Hit
GTGCCTCCTTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTA1770.7573164470306349No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA1610.6888584631182612No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATCACAGAGACCAAGTCTCTG1460.6246791032004108No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATCACAGAGACCAAGTCTCTG1160.49632038336470996No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1100.4706486393975698No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG870.37224028752353244No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC850.3636830395344857No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG770.32945404757829877No Hit
GTGCTGTCTCTCTTTGATGGAAGTAGACATGGGAGACTTTTCATTTTGTT630.26955331165497176No Hit
GTGCTGTCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAG590.2524388156768783No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG500.21393119972616803No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC480.20537395173712134No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCGGTCTCTC470.201095327742598No Hit
GCAAAAGTTCTCAGGGATGTCCAAGAGCAACTCTGCGTTGATACCACTGC390.16686633578641108Clontech SMART CDS Primer II A (95% over 22bp)
GCAAAAGTTCTCAGGGATGTACAAGAGCAACTCTGCGTTGATACCACTGC360.154030463802841Clontech SMART CDS Primer II A (95% over 22bp)
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCAGTCTCTC360.154030463802841No Hit
GTGCTGTCTCTCTTTGATGGATCGCTACAGGGCTCCTGGTGCTGAAGGAC360.154030463802841No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG350.14975183980831763No Hit
GTGCTGTCTCTCTTTGATGGGGTGCCTCCTTCTCTTTTGGTTGTCACAGA350.14975183980831763No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG330.14119459181927094No Hit
GTGCTGTCTCTCTTTGATGGATATGAAGCACCGCCAGGTCCTTTGAGTTT290.12408009584117749No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA290.12408009584117749No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATCACAGA280.11980147184665411No Hit
GGGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG280.11980147184665411No Hit
GTGCTGTCTCTCTTTGATGGAATCGAAAGGCGGGAAAGGTGGGGAAAAGA260.1112442238576074No Hit
GTGCCTCCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAG240.10268697586856067No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCGGCA240.10268697586856067No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG240.10268697586856067No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGACTCTC240.10268697586856067No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)