FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10345
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT427641.33397776703722No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT217120.98598356694055No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA10009.666505558240695No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA1661.6046399226679557No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC1251.208313194780087No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGT1071.0343160947317545No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC930.8989850169163847No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA750.7249879168680522Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG390.37699371677138716No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGACCCTGGGCTTCACTTACTCT370.35766070565490576No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT320.30932817786370226No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT270.2609956500724988No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC260.2513291445142581No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCATGTT250.2416626389560174No Hit
CAACCAAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGT250.2416626389560174No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATGTTG240.2319961333977767No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT220.2126631222812953No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG210.2029966167230546No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT210.2029966167230546No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA210.2029966167230546No Hit
GCATCAATCTTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT210.2029966167230546No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA200.1933301111648139No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC200.1933301111648139Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCGTCAGGGCAGTGGAAGCGTAGAGTGGG190.18366360560657322No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG190.18366360560657322No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT180.17399710004833252No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA180.17399710004833252Clontech Universal Primer Mix Long (96% over 26bp)
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT180.17399710004833252No Hit
TCCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT180.17399710004833252No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC170.16433059449009182No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG170.16433059449009182No Hit
GCATAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG160.15466408893185113No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA150.14499758337361043No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT140.13533107781536974No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG140.13533107781536974No Hit
CCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT130.12566457225712904No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCCTCACTTACTCT120.11599806669888835No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT120.11599806669888835No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT120.11599806669888835No Hit
TCCTATCAGTGGCCGCAAAGAGAGCTACTCCATCTACGTGTACAAGGTGC120.11599806669888835No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA120.11599806669888835No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACCCT110.10633156114064765No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA110.10633156114064765No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)