FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1654
Total Bases239.6 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT79548.065296251511484No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA1036.2273276904474No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA1026.166868198307134No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGT784.71584038694075No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA583.506650544135429Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC281.6928657799274487No Hit
GCATCAATCATCACAGGGTTGGGGGGTAAGGTCACAGATCAACAGGATCC241.4510278113663846No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC191.1487303506650544Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA171.0278113663845223Clontech Universal Primer Mix Long (96% over 26bp)
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT160.9673518742442563No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC160.9673518742442563Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC120.7255139056831923No Hit
CAACCAAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGT110.6650544135429262No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCATGTT90.5441354292623942No Hit
GCATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG80.48367593712212814Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA70.42321644498186217No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT70.42321644498186217No Hit
TCCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT60.36275695284159615No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT60.36275695284159615No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT50.3022974607013301No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC50.3022974607013301No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT50.3022974607013301No Hit
CAACCAAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTCAGTGTA40.24183796856106407No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA40.24183796856106407No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGTCCCTGGGCTTCACTTACTCT40.24183796856106407No Hit
GGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA40.24183796856106407No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT40.24183796856106407No Hit
GCATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG40.24183796856106407Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATGTTG30.18137847642079807No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATCAAAG30.18137847642079807No Hit
GCATCAATCATCACAGGGTTGGAAGCAGTGGTATCAACGCAGAGTACATG30.18137847642079807Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC30.18137847642079807Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG30.18137847642079807No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCTCTGGGCTTCACTTACTCT30.18137847642079807No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA30.18137847642079807No Hit
CAACCAAAAGAGAAGGAGGCACAAGATGAGTATTCAACATTTCCGTGTCC30.18137847642079807No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTCACTCT20.12091898428053204No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACGGCACCATCAAAGA20.12091898428053204No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCATGTT20.12091898428053204No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT20.12091898428053204No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.12091898428053204No Hit
CAACCAAAAGAGAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGTA20.12091898428053204No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGTGGCCCTGGGCTTCACTTACTCT20.12091898428053204No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT20.12091898428053204No Hit
GCATCAATCATCACAGGGTTGGCCGACCTGATGTCAACCTGCTTGGGTGA20.12091898428053204No Hit
CAACCAAAAGAGAAGGAGGCATCCATCAAAGAGAGACAGCACCCATGTTG20.12091898428053204No Hit
TCCTATCAGTGGCAGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT20.12091898428053204No Hit
TCCTATCAGTGGCCGCAAAGAGGCGAGAGGCCCTGGGCTTCACTTACTCT20.12091898428053204No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA20.12091898428053204Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGACTTCACTTACTCT20.12091898428053204No Hit
TCCTATCAGTGGCCGCAACGAGGGGAGAGGCCCTGGGCTTCACTTACTCT20.12091898428053204No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGAGCTTCACTTACTCT20.12091898428053204No Hit
TCCTATCAGCGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT20.12091898428053204No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGCTTCACTTACTCTT20.12091898428053204No Hit
TCCTATCAGTGGCCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTC20.12091898428053204No Hit
CAACAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAGAG20.12091898428053204No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT20.12091898428053204No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGTA20.12091898428053204No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACTATCAAAGA20.12091898428053204No Hit
CAACCAAAAGAGAAGGAGGCACGGAAATCAGTTTATGCATTCTCTAACAA20.12091898428053204No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA20.12091898428053204No Hit
CAACCAAAAGAGAAGGAGGCACGATTCCATCAAAGAGAGACAGCACCCAT20.12091898428053204No Hit
TCCTACCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT20.12091898428053204No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA20.12091898428053204No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG20.12091898428053204No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)