FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13100
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA584244.595419847328245No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA188314.374045801526716No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGC9517.259541984732825No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC3792.8931297709923665No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGTCACAGAGA3332.5419847328244276No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA2271.7328244274809161No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGATCACAGAGACCAAGTCTCTG1721.3129770992366412No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT1551.183206106870229No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC1421.083969465648855No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGATCACAGAGACCAAGTCTCTG1300.9923664122137404No Hit
GTGCCTCCTTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTA710.5419847328244275No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG680.5190839694656488No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG560.4274809160305344No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGATCACAGA560.4274809160305344No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG520.39694656488549623No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT470.35877862595419846No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA430.3282442748091603No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG410.31297709923664124No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA410.31297709923664124No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA380.2900763358778626No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA370.2824427480916031No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGATCACAGAGACCAAGTCTCTG340.2595419847328244No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA340.2595419847328244No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT300.22900763358778628Clontech Universal Primer Mix Long (96% over 26bp)
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT300.22900763358778628Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG270.20610687022900764No Hit
GTGCTGTCTCTCTTTGATGGGTGCCTCCTTCTCTTTTGGTTGTCACAGAG270.20610687022900764No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC250.19083969465648853No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC250.19083969465648853No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG240.183206106870229No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA230.17557251908396945No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA210.16030534351145037No Hit
CAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA200.15267175572519084No Hit
GGGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG190.1450381679389313No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCGGTCTCTC180.13740458015267176No Hit
GTGCTGTCTCTCTTTTGGTTGTCACAGAGACCAAGTCTCTGCTACCGTAG180.13740458015267176No Hit
GGTGTTTGGGATGGAATGGTGTTCCACGCAGGTGGTTCGCAGAAGCAGCA160.12213740458015268No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC160.12213740458015268No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCCTCCTTCTCTTTTGGTTGTCACAG150.11450381679389314No Hit
GAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGTGGTTCGCAGAAGCA150.11450381679389314No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)