FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4495
Total Bases651.4 kbp
Sequences flagged as poor quality0
Sequence length139-145
%GC55

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA141931.56840934371524No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGT85919.11012235817575No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1834.07119021134594Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGT1513.3592880978865405No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC942.0912124582869853No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC932.0689655172413794No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC641.4238042269187987Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA561.2458286985539488No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA491.0901001112347053No Hit
CAACCAAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTCAGTGTA461.0233592880978866No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA430.9566184649610678Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCATGTT390.8676307007786429No Hit
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATGTTG330.7341490545050056No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC280.6229143492769744Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG240.5339265850945495No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGTC240.5339265850945495No Hit
GCATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG230.5116796440489433Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT230.5116796440489433No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGCCATGTT210.46718576195773087No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGCCATGTTGTAGAACCATGT190.4226918798665183No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG190.4226918798665183No Hit
CAACCAAAAGAGAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGTA190.4226918798665183No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA180.40044493882091214Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATAAAAGA180.40044493882091214No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGCCATGTTGTAGA160.3559510567296997No Hit
CAACAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG150.33370411568409347No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGAAC150.33370411568409347No Hit
GCATCAATCATCACAGGGTTGGAAGCAGTGGTATCAACGCAGAGTACATG140.3114571746384872Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCTCAAAGA140.3114571746384872No Hit
CAACCAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGTA140.3114571746384872No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCAACAAAGA130.28921023359288095No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCCATGT130.28921023359288095No Hit
CAACCAAAAGAGAAGGGGGCACCATCAAAGAGAGACAGCACCATCAAAGA120.26696329254727474No Hit
CAACAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGTA110.2447163515016685No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGCCATGTTGTAGA110.2447163515016685No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATCAAAG100.22246941045606228No Hit
GCATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG90.20022246941045607Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCCCCATCAAAGAGAGACAGCACCATCAAAGA90.20022246941045607No Hit
CAACCAAAAGAGAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGTA90.20022246941045607No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCCCATGT80.17797552836484984No Hit
CAACCAAAAGAGAAGGGGGCACCATCAAAGAGAGACAGCACCCATGTTGT80.17797552836484984No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCGAAGA70.1557285873192436No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCCATCAAA70.1557285873192436No Hit
CACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG70.1557285873192436No Hit
AACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG70.1557285873192436No Hit
GCATCAATCATCACAGGGTTGGATTTGCCCTTTACCCTCTCAAGAGAGTT70.1557285873192436No Hit
AACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCCATGTTGTA60.13348164627363737No Hit
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCAATAA60.13348164627363737No Hit
GCATCAATCATCAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTC60.13348164627363737No Hit
CAACCAAAGAGAAGGAGGCACCCATGTTGTAGAACCATGTCGTCAGTGTA60.13348164627363737No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACGGCACCATCAAAGA60.13348164627363737No Hit
CAACCAAAAGAGAAGGAGGCACCCTCCATCAAAGAGAGACAGCACCCTCC60.13348164627363737No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGGACAGCACCCATGTTGTA50.11123470522803114No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGT50.11123470522803114No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATGTTGTA50.11123470522803114No Hit
CAACCAAAAGAGAAGGGGCACCATCAAAGAGAGACAGCACCATCAAAGAG50.11123470522803114No Hit
CAACCAAAAGAAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGAG50.11123470522803114No Hit
CAACCAAAAGAGAAGGAGGCACATCCATCAAAGAGAGACAGCACCCATGT50.11123470522803114No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACACACCCATGTTGTA50.11123470522803114No Hit
CAACCAAAGAGAGACAGCACCCATGTTGTAGAACCATGTCGTCAGTGTAG50.11123470522803114No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGCTGC50.11123470522803114Clontech Universal Primer Mix Long (95% over 23bp)
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGGA50.11123470522803114Clontech SMARTer II A Oligonucleotide (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)