FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29177
Total Bases4.2 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT901730.90447955581451No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTTCTGGATCCAGCCCCTGAC555119.025259622305242No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA443615.203756383452719No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT293710.06614799328238No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA19336.625081399732665No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGT2600.8911128628714399No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC990.3393083593241252No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC980.335881002159235No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCGAATCC970.3324536449943449No Hit
CAACAAAAGAGAAGGAGGCACCACAGGTTTTCTGGATCCAGCCCCTGACA780.26733385886143196No Hit
CAACCAAAGAGAAGGAGGCACCACAGGTTTTCTGGATCCAGCCCCTGACA770.26390650169654184No Hit
CAACAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG760.2604791445316516No Hit
CAACCAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG660.22620557288275012No Hit
CAACCAAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTA630.21592350138807964No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT600.20564142989340917No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA600.20564142989340917No Hit
CAACCAAAAGAGAGGAGGCACCACAGGTTTTCTGGATCCAGCCCCTGACA580.1987867155636289No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT520.17822257257428797No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC460.15765842958494702No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA430.14737635809027658No Hit
CAACCAAAAGAGAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGTA420.14394900092538643No Hit
CAACCAAAAGAGAAGGAGGCCCCACAGGTTTTCTGGATCCAGCCCCTGAC360.1233848579360455No Hit
CAACCAAAAGAGAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG360.1233848579360455No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA340.11653014360626521No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACCTACTCT330.11310278644137506No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG330.11310278644137506No Hit
CACCAAAAGAGAAGGAGGCACCACAGGTTTTCTGGATCCAGCCCCTGACA330.11310278644137506No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT320.1096754292764849No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA320.1096754292764849No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT310.10624807211159476No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)