FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18963
Total Bases2.7 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT899747.44502452143648No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA393320.740389178927384No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA191910.11970679744766No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGT3281.7296841217107No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC1150.6064441280388124No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1030.5431630016347624Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TCCTATCAGTGGCCGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC1000.5273427200337499No Hit
CAACCAAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTA950.5009755840320624No Hit
CAACAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG650.34277276802193746No Hit
CAACCAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG630.33222591362126247No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT570.30058535041923745No Hit
CAACCAAAAGAGAAGGAGGCACTAGGGATCGTCCGCAGGATTGGGACTGA540.28476506881822494No Hit
CAACCAAAAGAGAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGTA540.28476506881822494No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT500.26367136001687497No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT480.2531245056162No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC470.24785107841586246No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA430.22675736961451248No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT410.2162105152138375No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA400.21093708801349997No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA360.18984337921214997No Hit
CAACCAAAAGAGAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG340.17929652481147498No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGGAATCCTGTAGA330.1740230976111375No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCGAATCC310.16347624321046247No Hit
CACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG310.16347624321046247No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT300.15820281601012498No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT290.1529293888097875No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA290.1529293888097875No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGT270.14238253440911247No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT260.13710910720877498No Hit
CAACCAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTAG250.13183568000843748No Hit
CAACCAAAAGAGAAGGAGGCACAGTCACACGATGAGTGTGTGTGGATGGG250.13183568000843748No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT220.116015398407425No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT220.116015398407425No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGGAATCCT220.116015398407425No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT210.11074197120708748No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGGAATCCTGTAGAAC200.10546854400674999No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCGCTTACTCT200.10546854400674999No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGACTTCACTTACTCT200.10546854400674999No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT190.1001951168064125No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA190.1001951168064125No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)