FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26634
Total Bases3.8 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT1238546.50071337388301No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATTGCGAGATGTCCCTCTT448116.824359840804988No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT318111.943380641285575No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA14215.335285725013141No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC1460.5481715100998723No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA1110.4167605316512728No Hit
CAACCAAAAGAGAAGGAGGCACCCAATGTGTGGTAGTTATTATTATCTTC1030.3867237365773072No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT990.37170533904032443No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT900.3379139445821131No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGT890.3341593451978674No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC880.33040474581362167No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT480.18022077044379364No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT480.18022077044379364No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATTGCGAGATGTCCCTCTTG440.16520237290681083No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT420.15769317413831943No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT400.15018397536982805No Hit
CAACCAAAAGAGAAGGAGGCACCCAATAGCAGCAGAGAGACGGAGCCCCC390.14642937598558234No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC360.13516557783284522No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATTGCGAGATGTCCCTCTTG340.12765637906435384No Hit
GCATCAATCATCACAGGGTTGGTATCAACGCAGAGTTGCTCCTTCTCCAT330.12390177968010813No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG330.12390177968010813No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA330.12390177968010813No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT320.12014718029586242No Hit
GCATCAATCTCACAGGGTTGGACTCGAGAAATTGCGAGATGTCCCTCTTG320.12014718029586242No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT320.12014718029586242No Hit
CAACCAAAAGAGAAGGAGGCACACGCCCGCTTGCTGGGAGAGAGGCTGAG320.12014718029586242No Hit
CAACCAAAAGAGAAGGAGGCACTGCCCGCCTGCCTGCTCCGAGACGTGGC320.12014718029586242No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT320.12014718029586242No Hit
TCCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT320.12014718029586242No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATTGCGAGATGTCCCTCTTG300.11263798152737103No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT290.10888338214312533No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCGAATCC270.10137418337463391No Hit
CAACCAAAAGAGAAGGAGGCACCGCCTGCCCAGTGACACATGTTTAACGG270.10137418337463391No Hit
GCATCAATCATCACAGGGTTGGTTCGGCTGATCTGGCTGGCTAGGCGGGT270.10137418337463391No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG270.10137418337463391No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT270.10137418337463391No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)