FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23596
Total Bases3.4 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA527522.35548398033565No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA343814.570266146804542No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA332214.078657399559248No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG16717.081708764197321No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC15996.776572300389897No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCAT10544.46685878962536No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT3441.4578742159688083Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC3261.3815901000169521No Hit
GTGCCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTA2661.1273097135107646No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT2120.8984573656551957Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC1670.7077470757755552Clontech SMART CDS Primer II A (95% over 24bp)
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC1480.6272249533819292No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGCTGCATAGA1460.6187489404983896No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT1120.47465672147821664No Hit
GTGCTGTCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAC1110.47041871503644683No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGACTGCATAGACCAAGTCTCTG1060.44922868282759787No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT1050.4449906763858281No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGACTGCATAGACCAAGTCTCTG1010.4280386506187489No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCATGTA1000.42380064417697916Clontech SMART CDS Primer II A (96% over 26bp)
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTTCATGGTTTA850.36023054755043227No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA840.3559925411086625No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGACCCCCATG640.27123241227326667No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG580.2458043736226479No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG550.23309035429733854No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA540.22885234785556874No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT510.21613832853025938No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA460.19494829632141042No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT450.19071028987964062No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGATACCCCCA420.17799627055433126No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA420.17799627055433126No Hit
GTGCCTCCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAC370.1568062383454823No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGCTGCAT340.1440922190201729No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA340.1440922190201729No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC330.1398542125784031No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGATCCCCCAT320.13561620613663333No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGAGACCCCCA300.12714019325309373No Hit
GCAAAAGTTCTCAGGGATGTCCGAGAGCTGCCCATGTACTCTGCGTTGAT290.12290218681132395No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCATGTA280.11866418036955416Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGTCATCCCCAT280.11866418036955416No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGGACCCCCAT270.11442617392778437No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG270.11442617392778437No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT270.11442617392778437Clontech SMART CDS Primer II A (96% over 25bp)
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCCTGCATAGACCAAG270.11442617392778437No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCCTGCATAGACCA260.11018816748601457No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGACTGCATAGACCAAGTCTCTG260.11018816748601457No Hit
GTGCTGTCTCTCTTTGATGGATCGCTACAGGGCTCCTGGTGCTGAAGGAC260.11018816748601457No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGACCCCATGT250.10595016104424479No Hit
GCAAAAGTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT250.10595016104424479No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG250.10595016104424479No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGTA240.10171215460247499No Hit
GCAAAAGTTTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT240.10171215460247499No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)