FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27725
Total Bases4 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG879131.70784490532011No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA797128.750225428313797No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA544519.63931469792606No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA18396.633002705139765No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT2170.7826871055004508No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1040.37511271415689806No Hit
GTGCCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTA900.3246167718665464No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG860.3101893597835888No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT750.2705139765554554No Hit
GCAAAAGTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT530.19116321009918844No Hit
GAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT490.17673579801623082No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC470.16952209197475201No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG430.1550946798917944No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG410.1478809738503156No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGACTGCATAGACCAAGTCTCTG380.13706041478809738No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGACTGCATAGACCAAGTCTCTG360.12984670874661858No Hit
GCAAAAGTTTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT350.12623985572587917No Hit
GTGCTGTCTCTCTTTGATGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC340.12263300270513977No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA310.11181244364292156No Hit
GGGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG310.11181244364292156No Hit
GCAAAAGTTCTCGGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG300.10820559062218214No Hit
GCAAAAGTTCTCAGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT290.10459873760144274No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG290.10459873760144274No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA290.10459873760144274No Hit
GCAAAAGTTCTCGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT280.10099188458070334No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)