FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22409
Total Bases3.2 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT868738.76567450577893No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT472421.080815743674417No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA263811.772055870409211No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA241710.78584497300192No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC760.33914944888214554No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGT720.32129947788834845No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA650.29006202864920344No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC630.28113704315230487No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT600.26774956490695706No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT600.26774956490695706No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG580.2588245794100585No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA490.2186621446740149No Hit
CAACAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG480.21419965192556562No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT450.20081217368021778No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA420.18742469543486992No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT390.1740372171895221No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC340.15172475344727565No Hit
CAACCAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG340.15172475344727565No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA320.14279976795037708No Hit
CAACCAAAAGAGAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG320.14279976795037708No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG320.14279976795037708No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT300.13387478245347853No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCGAATCC290.12941228970502924No Hit
GCATAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG290.12941228970502924No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG280.12494979695657994No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT280.12494979695657994No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA280.12494979695657994No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACCTACTCT270.12048730420813067No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG250.1115623187112321No Hit
GCATCAATCATCACGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG240.10709982596278281No Hit
GCATCAATCTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG230.10263733321433353No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT230.10263733321433353No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)