FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20585
Total Bases2.9 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA747436.30799125576876No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT691533.59242166626184No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA16397.9621083313092065No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGT1720.8355598736944377No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA1500.7286859363614282No Hit
CAACCAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG1180.5732329366043235No Hit
CAACAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG1140.5538013116346855No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC1110.5392275929074569No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC950.4615010930289046No Hit
CAACCAAAAGAGAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG700.3400534369686665No Hit
CAACCAAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTA670.32547971824143795No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT600.2914743745445713No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT550.2671848433325237No Hit
CAACCAAAAGAGAAGGAGGCACACTAGGGGGGGGGGGGGGGGGGGGGGGG480.23317949963565704No Hit
CACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG480.23317949963565704No Hit
CAACCAAAAGAGAAGGAGGCACTAGGGATCGTCCGCAGGATTGGGACTGA470.2283215933932475No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC470.2283215933932475No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCGAATCC450.21860578090842847No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC400.19431624969638087No Hit
CAACCAAAAGAGAAGGAGGCCCCACAGGTTTCTGGATCCAGCCCCTGACA390.18945834345397136No Hit
CAACCAAAAGAGAAGGAGGCACGCTGAAAGGAAACATCAGTGCTCATCGC370.1797425309691523No Hit
AACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG340.16516881224192373No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGGAATCCTGTAGAAC330.1603109059995142No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA330.1603109059995142No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACGAATCCTGT320.1554529997571047No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGGAATCCT310.15059509351469516No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT300.14573718727228566No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT300.14573718727228566No Hit
CAACCAAAAGAGAAGGAGCACCACAGGTTTCTGGATCCAGCCCCTGACAG280.1360213747874666No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA270.1311634685450571No Hit
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACG260.12630556230264756No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA260.12630556230264756No Hit
CAACCAAAAGAGAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGTA260.12630556230264756No Hit
CAACCAAAAGAAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACAG250.12144765606023804No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGT240.11658974981782852No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT230.11173184357541899No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGGAATCCTGTAGA230.11173184357541899No Hit
GCATCAATCATCACAGGGTTGGCAAGATGTGGACATCCCTGAGAACTTTT220.10687393733300947No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)