FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14515
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT985367.8815018945918No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA11978.246641405442645No Hit
CAACCAAAAGAGAAGGAGGCACTCATCGTCCCTCACAGCAGCCGCCGCCG2901.9979331725800895No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2891.9910437478470548Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGT1190.8198415432311402No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA1030.7096107475025836No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC770.5304857044436789No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC700.48225973131243544No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC690.4753703065794006Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT650.44781260764726144No Hit
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT590.40647605924905267No Hit
TCCTTCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT430.296245263520496No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT400.2755769893213917No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA350.24112986565621772Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTATCAGTGGCCGCAAAGAGGGGAGGGGCCCTGGGCTTCACTTACTCT320.22046159145711333No Hit
TCCTATCGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT310.21357216672407853No Hit
CAACCAAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTA300.20668274199104375No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC290.19979331725800895No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCCTCACTTACTCT280.19290389252497417No Hit
TCCTATCATGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT270.1860144677919394No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA260.1791250430589046No Hit
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT240.165346193592835No Hit
TCCTATCAGTGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT240.165346193592835No Hit
TCCTACAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT230.1584567688598002No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACGAATCCTGT230.1584567688598002No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGTCCTGGGCTTCACTTACTCT230.1584567688598002No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGT220.1515673441267654No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGGAATCCTGTAGAAC210.14467791939373062No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA200.13778849466069584No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACCTACTCT200.13778849466069584No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGACTTCACTTACTCT200.13778849466069584No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCGAATCC190.13089906992766104No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA190.13089906992766104No Hit
CAACCAAAAGAGAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGTA180.12400964519462625No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCCGGGCTTCACTTACTCT170.11712022046159146No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA170.11712022046159146No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTCCACTTACTCT160.11023079572855667No Hit
GCATCATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC160.11023079572855667Clontech Universal Primer Mix Long (96% over 26bp)
TCCATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT160.11023079572855667No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGACCCTGGGCTTCACTTACTCT160.11023079572855667No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGGAATCCTGTAGA150.10334137099552188No Hit
GCATCAATCATCACAGGGTTGGGGTCATCCTCCCGCATGGTGCGTCGATC150.10334137099552188No Hit
TCCTATCAGTGGCCGCAAAGAGGGGGGAGGCCCTGGGCTTCACTTACTCT150.10334137099552188No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACCCT150.10334137099552188No Hit
TCCTATCAGTGGCCGCAAAGGGGGGAGAGGCCCTGGGCTTCACTTACTCT150.10334137099552188No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)