FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12455
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA660653.038940184664796No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA268421.549578482537136No Hit
GTGCCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTA1090.8751505419510237No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGCTGCAT1000.8028904054596547No Hit
GCAAAAGTTCTCAGGGATGTCCTCCTTCTCTTTTGGTTGCTGCATAGACC1000.8028904054596547No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGACTGCATAGACCAAGTCTCTG990.7948615014050583No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGACTGCATAGACCAAGTCTCTG840.67442794058611No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG770.6182256122039342No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG740.5941389000401446No Hit
GCAAAAGTTCTCAGGGATGTCCGGAGCCCAGGTCAGGAGACAGAGTTGTA570.45764753111200324No Hit
GCAAAAGTTCTCAGGGATGTGAAGCACCGCCAGGTCCTTTGAGTTTTAAG480.38538739462063426No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA470.37735849056603776No Hit
GCAAAAGTTCTCAGGGATGGCCTCCTTCTCTTTTGGTTGCTGCATAGACC430.34524287434765155No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG420.337213970293055No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA380.3050983540746688No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG380.3050983540746688No Hit
GTGCTGTCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAC380.3050983540746688No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGACTGCATAGACCAAGTCTCTG340.27298273785628263No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG330.264953833801686No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA310.24889602569249297No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC300.24086712163789645No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG300.24086712163789645No Hit
GCAAAAGTTCTCAGGGATGTCCCTTTCCTCAGCTTTCAGGCAGCACCCAC260.20875150541951024No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA240.19269369731031713No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCCTGCATAGACCAAG240.19269369731031713No Hit
GCAAAAGTTCTCAGGGATGTCCTTCTGTGGCCGCGCATGTTTCTCTGTGG210.1686069851465275No Hit
GCAAAAGTTCTCAGGGATGTCCGAAGAGAAAGTCAAAAGGTGAAGACAAA210.1686069851465275No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGACTGCATA210.1686069851465275No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCCTGCATAGACCA190.1525491770373344No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG190.1525491770373344No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGACTGCCTCCTTCTCTTTTGGTTG190.1525491770373344No Hit
GTGCCTCCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAC190.1525491770373344No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA190.1525491770373344No Hit
GCAAAAGTTCTCAGGGATGTCCCTTTACATATGTCCAATCTGGCTAATGC190.1525491770373344No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGTA190.1525491770373344No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCCTGCATAGACCAAGTCTCT180.14452027298273784No Hit
GCAAAAGTTCTCAGGGATGTCCGGGCAGGTGCCCACGATCTGCAGCTCCT180.14452027298273784No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA180.14452027298273784No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA170.13649136892814132No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA170.13649136892814132No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCCTGCATAGACCA160.12846246487354476No Hit
GCAAAAGTTCTCAGGGATGTCCCTAACTGTCCCCTCCAAGTGGTGGCACA160.12846246487354476No Hit
GCAAAAGTTCTCAGGGATGTCCGAGATGGCAGATGTGGCTACAGACATGT160.12846246487354476No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGTAGCA140.11240465676435167No Hit
GCAAAAGTTCTCAGGGATGTCGTTGCGCACAATCTGCTTGTGCTGGGTGA140.11240465676435167No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCGGGTGGTTCGCAGAAGCAGCA140.11240465676435167No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG140.11240465676435167No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCGGCA140.11240465676435167No Hit
GTGCTGTCTCTCTTTGATGGACCCACACCATTATCAGTGGTGTGGTTGGT140.11240465676435167No Hit
GTGCTGTCTCTCTTTGATGGACACCGAGCACGCGCCGCTTGCAGCTGGCT130.10437575270975512No Hit
GGTGTTTGGGATGGAATGGTGGTCCACGCAGGTGGTTCGCAGAAGCAGCA130.10437575270975512No Hit
GGGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG130.10437575270975512No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)