FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24808
Total Bases3.5 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA746730.09916156078684No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA589523.762495969042245No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA457618.445662689455013No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCAT22629.118026443082876No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCCGGTGCTGAAGGA4101.6526926797807158No Hit
GTGCCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTA1230.4958078039342148No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1160.46759109964527573No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1040.41921960657852303No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGACTGCATAGACCAAGTCTCTG870.3506933247339568No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA830.3345694937117059No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA790.318445662689455No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGACTGCATAGACCAAGTCTCTG650.2620122541115769No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC620.24991938084488874No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA510.2055788455336988No Hit
GTGCTGTCTCTCTTTGATGGATCGCTACAGGGCTCCTGGTGCTGAAGGAC500.2015478877781361No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA500.2015478877781361No Hit
GCAAAAGTTCTCAGGGATGTCCGTGGATTTACTTTCTACTTTGCAGAAGA490.19751693002257337No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA490.19751693002257337No Hit
GCAAAAGTTCTCAGGGATGTCCCATAAATGCTTTCAATAATTGTTTTGGT490.19751693002257337No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA480.19348597226701064No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGCTGCAT460.1854240567558852No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG440.17736214124475974No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG390.15720735246694614No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG350.14108352144469527No Hit
GCAAAAGTTCTCAGGGATGTCCCAGCGCTGCTCCCACTGCACCAGGCTCG340.13705256368913254No Hit
GTGCTGTCTCTCTTTGATGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC330.13302160593356982No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGACTGCATAGACCAAGTCTCTG310.12495969042244437No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG300.12092873266688164No Hit
GCAAAAGTTCTCAGGGATGTCTGTGGATTTACTTTCTACTTTGCAGAAGA280.1128668171557562No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG270.10883585940019348No Hit
GTGCTGTCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAG270.10883585940019348No Hit
GCAAAAGTTCTCAGGGATGTCCTGTTCCATTGGTCTATATCTCTGTTTTG260.10480490164463076No Hit
GCAAAAGTTCTCAGGGATGTCTAGAGCAGGTAACTGAAACCTTCACGTCT250.10077394388906805No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)