FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12134
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA393732.446019449480794No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA232119.128069886269987No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA143711.842755892533377No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG11709.642327344651394No Hit
GTGCCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTA2752.266358991264216No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC1140.9395088181968023No Hit
GTGCTGTCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAT1010.8323718477006757No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGACTGCATAGACCAAGTCTCTG1000.8241305422778968No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGACTGCATAGACCAAGTCTCTG860.7087522663589912No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG620.510960936212296No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG590.4862370199439591No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA530.4367891874072853No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGACTGCATAGACCAAGTCTCTG450.3708587440250536No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT410.3378935223339377No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCCTGCATAGACCAAGTCTCT340.2802043843744849No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA340.2802043843744849No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT330.27196307895170596No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGCTGCAT320.263721773528927No Hit
GTGCCTCCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAT320.263721773528927No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC310.255480468106148No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA310.255480468106148No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG300.24723916268336904No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCCTGCATAGACCAAG290.23899785726059006No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT260.21427394099225316No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA260.21427394099225316No Hit
GCAAAAGTTCTCAGGGATGTCCCAGCACAGCTCCTCAAACCCACTGTAGC250.2060326355694742No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGACTGCATA230.18955002472391627No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGCTGCATAGA230.18955002472391627No Hit
GTGCTGTCTCTCTTTGATGGAATCAAATCTCCCATCTCTTTATTCACAGG220.18130871930113732No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG200.16482610845557935No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG190.1565848030328004No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG190.1565848030328004No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA190.1565848030328004No Hit
GTGCTGTCTCTCTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTATC170.14010219218724246No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCCTGCATAGACCA170.14010219218724246No Hit
GTGCTGTCTCTCTTTGATGGACACTGACGACATGGTTCTACAGGATTCGT150.12361958134168452No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC150.12361958134168452No Hit
GTGCCTCCTTCTCTTTTGTTGCTGCATAGACCAAGTCTCTGCTACCGTAT140.11537827591890555No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG140.11537827591890555No Hit
GCAAAAGTTCTCAGGGATGTCCTCCTTCTCTTTTGGTTGCTGCATAGACC130.10713697049612658No Hit
GTGCTGTCTCTCTTTGATGGAATCCCCAATAAAGACGGTGGCCCAAACCT130.10713697049612658No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)