FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6445
Total Bases933.6 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA231335.88828549262995No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC4657.21489526764934No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTCTGCGTTGATACCA4056.283941039565555No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC2483.8479441427463152No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA2113.2738557020946466No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT1762.730799069045772Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTA1442.234290147401086No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT1281.986035686578743Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAAGTAACGGTGCTGCCACTGATAGGACTGCATAGACCAAGTCTCTG911.4119472459270752No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGACTGCATAGACCAAGTCTCTG861.334367726920093No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGCTGCATAGA761.1792086889061288No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT490.7602792862684251No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC470.7292474786656322Clontech SMART CDS Primer II A (95% over 24bp)
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT460.7137315748642358No Hit
GTGCTGTCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAA430.6671838634600465No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCCTGCATAGACCAAG430.6671838634600465No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC410.6361520558572537No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA350.5430566330488751No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGACTGCATAGACCAAGTCTCTG330.5120248254460822No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA330.5120248254460822No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCCTGCATAGACCAAGTCTCT280.4344453064391001No Hit
GTGCCTCCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAA230.3568657874321179No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA230.3568657874321179No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGACTGCATA220.3413498836307215No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCATGTA190.2948021722265322Clontech SMART CDS Primer II A (96% over 26bp)
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTTCATGGTTTA180.27928626842513576No Hit
GTGCTGTCTCTCTTTGAGGGTCATCCCCAAGCAGCTCTGCGTTGATACCA180.27928626842513576No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCAACCCTGTGATGATTGATGCCTG170.2637703646237393No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA170.2637703646237393No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCCTGCATAGACCA160.24825446082234287No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA160.24825446082234287No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA150.23273855702094648No Hit
GTGCTGTCTCTCTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAAC150.23273855702094648No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCGGGGCTCCTGGTGCTGA140.21722265321955006No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCCTGCATAGACCA130.20170674941815359No Hit
GTGCTGTCTCTCTTTGATGGACACTGACGACATGGTTCTACAGGATTCGT120.1861908456167572No Hit
GTGCTGTCTCTCTTTGATGGAATCCCCAAGCAGCTCTGCGTTGATACCAC120.1861908456167572No Hit
CTCCAAGAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA120.1861908456167572No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGACCCCCATG120.1861908456167572No Hit
GTGCTGTCTCTCTTTGATGGAATCTGCGTTGATACCACTGCTTCCCATGT90.13964313421256788Clontech Universal Primer Mix Long (95% over 23bp)
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGCGCAGGGCTCCTGGTGCTGA90.13964313421256788No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGACTCCTGGTGCTGA90.13964313421256788No Hit
CTCCAAGTAACGGTGCTTTCTCTTCACCCTTTGCCCCTGCATAGACCAAG90.13964313421256788No Hit
GTGCCTCCTTCTCTTTTGTTGCTGCATAGACCAAGTCTCTGCTACCGTAA80.12412723041117144No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGCTGCAT80.12412723041117144No Hit
GTGCTGTCTCTCTTTGATGGAATCCTGACGACATGGTTCTACAGGATTCG80.12412723041117144No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTATC80.12412723041117144No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAACTCTGCGTTGATACCA80.12412723041117144No Hit
GTGCTGTCTCTCTTTGATGGATAACGAGGGTGAGAGGCTCCCCGGGGTGG70.10861132660977503No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTA70.10861132660977503No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCCATGTACTCTGCGTTGATA70.10861132660977503No Hit
GCAAAAGTTCTCAGGGATGTGAGGCCCACCCATGTACTCTGCGTTGATAC70.10861132660977503No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT70.10861132660977503Clontech SMART CDS Primer II A (96% over 25bp)
GTGCTGTCTCTCTTTGATTGCTGCATAGACCAAGTCTCTGCTACCGTAAC70.10861132660977503No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTCT70.10861132660977503No Hit
GCAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATACC70.10861132660977503No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTTGCGTTGATACCA70.10861132660977503No Hit
GCAAAAGTTCTCAGGGATGTGCGTTGATACCACTGCTTCCCATGTACTCT70.10861132660977503Clontech Universal Primer Mix Long (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)