FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10641
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length136-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT293427.572596560473638No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA139313.090874917770886Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA8057.5650784700686025No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT7406.954233624659337No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC3793.561695329386336Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGT2051.926510666290762No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC1541.4472324029696457No Hit
TCCTATCAGTGGCAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC1421.334461046894089No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA1411.3250634338877925No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1301.2216896908185322Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGAAGCA1071.005544591673715Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1020.9585565266422329Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCACAGGTTTCTGGATCCAGCCCCTGACA1000.93976130062964No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGAAGC990.9303636876233436Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGGCAAAGGGTGAAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC800.7518090405037121Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACGAATCCTGT630.5920496193966732No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC580.5450615543651912No Hit
GCATCAATCATCACAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC470.4416878112959309Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGGTGACAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGT470.4416878112959309No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGGAATCCT360.33831406822667043No Hit
CAACCAAAAGAGAAGGAGGCACCAAAGGTTAGAATATGGGGTGTCAGATT360.33831406822667043No Hit
GGGCAAAGGGTGAAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA360.33831406822667043Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACCTCCAT340.3195188422140776No Hit
CAACCAAAAGAGAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGTA340.3195188422140776No Hit
CAACCAAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTA310.2913260031951884No Hit
GCATCATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTACA250.23494032515741Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACCAAAAGAGAAGGAGGCACCCATCAAAGAGAGACAGCACCCATCAAA250.23494032515741No Hit
GCATAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC240.22554271215111363Clontech Universal Primer Mix Long (96% over 26bp)
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGGA240.22554271215111363Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT230.21614509914481722No Hit
GCATCAATCATCACAGGGTTGGTCCTCACATCCCTGAGAACTTTTGCGAA230.21614509914481722No Hit
CAACCAAAAGAGAAGGAGGCACCGTCCATCAAAGAGAGACAGCACCGTCC220.20674748613852081No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGGAATCCTGTAGA220.20674748613852081No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGCTGC210.19734987313222443No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGGAATCCTGTAGAAC210.19734987313222443No Hit
CAACCAAAAGAGAAGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC190.17855464711963162Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGGGTTGGTATCAACGCAGAGTACATGGGAAGCAGT180.16915703411333521No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA180.16915703411333521No Hit
GATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC170.1597594211070388Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATCACAGGGTTCATGGGAAGCAGTGGTATCAACGCAGAGTA160.1503618081007424Clontech Universal Primer Mix Long (96% over 26bp)
CAACCAAAAGAGAAGGAGGCACCCCATCAAAGAGAGACAGCACCCCATCA160.1503618081007424No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGGAATCCTGTAGA150.140964195094446No Hit
TCCTATCAGTGGCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT150.140964195094446No Hit
GCATCATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC140.13156658208814961Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCTATCAGTGGCCGCAAGGAGGGGAGAGGCCCTGGGCTTCACTTACTCT140.13156658208814961No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA140.13156658208814961No Hit
GCATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG140.13156658208814961Clontech Universal Primer Mix Long (96% over 26bp)
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA130.12216896908185321No Hit
CAACCAAAAGAGAAGGAGGCACCTCCATCAAAGAGAGACAGCACGAATCC130.12216896908185321No Hit
GCATCAATCATCACAGGGTTGGGAACAGCTCGTACACTTGCCATTCTCTG130.12216896908185321No Hit
CAACCAAAAGAGAAGGAGGCAGTGGTATCAACGCAGAGTACATGGGTGGT130.12216896908185321Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATCAATCATCACAGGGTACATGGGAATCAGTGGTATCAACGCAGAGTA130.12216896908185321Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCATCAATCATCACAGGGTACATGGGAGCAGTGGTATCAACGCAGAGTAC120.11277135607555681Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACCAAAAGAGAAGGAGGCACGCGCGCGGCTGAGGCGAGGTCGCTCGGC120.11277135607555681No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA120.11277135607555681No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGCGTA120.11277135607555681Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAACCAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTAG110.10337374306926041No Hit
CAATCAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTAG110.10337374306926041No Hit
TCCTATAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCTT110.10337374306926041No Hit
GCATCAATCATCACAGGGTACATGGGGAGCAGTGGTATCAACGCAGAGTA110.10337374306926041Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CATCAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTAGA110.10337374306926041No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)