FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17671
Total Bases2.5 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA785544.45136098692773No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA17589.948503197328957No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC7734.374398732386395No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGATCCAGGAGTGGGGCCCAT7484.23292399977364No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT3021.7090147699620848No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT2771.5675400373493293Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT2441.3807933903004923Clontech Universal Primer Mix Long (96% over 26bp)
GTGCCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTA2371.341180465168921No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA2121.1997057325561655No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC1831.035595042725369No Hit
GTGCTGTCTCTCTTTGATGGAATCCATGCCTGAATCTGCGGTATACAATT1750.9903231282892876No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC1290.7300096202818177Clontech SMART CDS Primer II A (95% over 24bp)
CTCCAAGTAACGGTGCTGCCACTGATAGGACTGCATAGACCAAGTCTCTG1220.6903966951502462No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGACTGCATAGACCAAGTCTCTG1160.6564427593231849No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1070.6055118555825929No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC990.5602399411465112No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG960.5432629732329806No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGCTGCATAGA790.4470601550563069No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG540.3055854224435516No Hit
GCAAAAGTTCTCAGGGATGTGGAATACTTAGCAACTCTGCGTTGATACCA540.3055854224435516No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT510.28860845453002093No Hit
GCAAAAGTTCTCAGGGATGTCTGCGTTGATACCACTGCTTCCCATGTACT440.2489955293984494Clontech Universal Primer Mix Long (95% over 24bp)
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG430.2433365400939392No Hit
GTGCTGTCTCTCTTTGATGGAGGTCATCCCCAAGCAGCTGCGTTGATACC410.23201856148491878No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGACTGCATAGACCAAGTCTCTG390.22070058287589836No Hit
GTGCTGTCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAA380.21504159357138816No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGACTGCATA380.21504159357138816No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTCTGCGTTGATACCA370.20938260426687796No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT350.1980646256578575Clontech SMART CDS Primer II A (96% over 25bp)
GTGCTGTCTCTCTTTGATGGAATCTGCGTTGATACCACTGCTTCCCATGT340.19240563635334731Clontech Universal Primer Mix Long (95% over 23bp)
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG340.19240563635334731No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG330.1867466470488371No Hit
GTGCTGTCTCTCTTTGATGGACTCTGCGTTGATACCACTGCTTCCCATGT310.17542866843981664Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCCTGCATAGACCAAGTCTCT300.16976967913530644No Hit
GTGCCTCCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAA300.16976967913530644No Hit
GCAAAAGTTCTCAGGGATGTGGAATACTTAGCAACTCTGCGTTGATACCC280.15845170052628602No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA270.1527927112217758No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG270.1527927112217758No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCATGTA260.1471337219172656Clontech SMART CDS Primer II A (96% over 26bp)
GCAAAAGTTCTCAGGGATGTGCGTTGATACCACTGCTTCCCATGTACTCT250.14147473261275537Clontech Universal Primer Mix Long (95% over 21bp)
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA250.14147473261275537No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG250.14147473261275537No Hit
GTGCTGTCTCTCTTTGATGGACCTAGGCTTGACCCATGTACTCTGCGTTG230.13015675400373491No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC230.13015675400373491No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA220.1244977646992247No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA210.11883877539471452No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGTA210.11883877539471452No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA210.11883877539471452No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTTGCGTTGATACCA210.11883877539471452No Hit
GGGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG200.11317978609020429No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCAACCCTGTGATGATTGATGCCTG200.11317978609020429No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGATACCCCCA180.10186180748118386No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG180.10186180748118386No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC180.10186180748118386No Hit
GTGCTGTCTCTCTTTGATGCCACTGCTTCCCATGTACTCTGCGTTGATAC180.10186180748118386No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCCTGCATAGACCAAG180.10186180748118386No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)