FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6366
Total Bases922.3 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA279243.85799560163368No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC3906.126295947219605No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTG3164.963870562362551No Hit
GTGCCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTA2003.1416902293433866No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT1852.906063462142633No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT1061.6650958215519949Clontech Universal Primer Mix Long (96% over 26bp)
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT851.3352183474709394Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC731.1467169337103362No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC671.0524662268300347Clontech SMART CDS Primer II A (95% over 24bp)
CTCCAAGTAACGGTGCGGCCACTGATAGGACTGCATAGACCAAGTCTCTG661.0367577756833177No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGACTGCATAGACCAAGTCTCTG651.0210493245366006No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC610.9582155199497331No Hit
GTGCTGTCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAA420.6597549481621112No Hit
GTGCTGTCTCTCTTTGATGGACGGGAATCTAGGTACACCCATGTACTCTG410.6440464970153943No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTCTGCGTTGATACCA310.48696198554822495No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGCTGCATAGA310.48696198554822495No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG270.4241281809613572No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGACTGCATAGACCAAGTCTCTG260.4084197298146403No Hit
GTGCCTCCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAA230.36129437637448947No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG210.3298774740810556No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG190.2984605717876217No Hit
GTGCTGTCTCTCTTTGATGGAGGTCATCCCCAAGCAGCTGCGTTGATACC170.2670436694941879No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGACTGCATA170.2670436694941879No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG160.2513352183474709No Hit
GGGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG160.2513352183474709No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT150.23562676720075398No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCATGTA150.23562676720075398Clontech SMART CDS Primer II A (96% over 26bp)
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCCTGCATAGACCAAGTCTCT140.21991831605403708No Hit
GTGCTGTCTCTCTTTGATGGAGGTGCTGTCTCTCTTTGATGGAGGTGCTG140.21991831605403708No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCAACCCTGTGATGATTGATGCCTG140.21991831605403708No Hit
GTGCTGTCTCTCTTTGATGGATGAAGAGGAAACCAGGATGTGCAGCCAAG140.21991831605403708No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG130.20420986490732015No Hit
GTGCTGTCTCTCTTTGATGGACCTAGGCTTGACCCATGTACTCTGCGTTG130.20420986490732015No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCCTGCATAGACCA120.1885014137606032No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCCTGCATAGACCAAG120.1885014137606032No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC120.1885014137606032No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT100.15708451146716934No Hit
GCAAAAGTTCTCAGGGATGTCCCCATGTACTCTGCGTTGATACCACTGCT100.15708451146716934Clontech SMART CDS Primer II A (96% over 25bp)
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG100.15708451146716934No Hit
GTGCTGTCTCTCTTTGATGCTGCATAGACCAAGTCTCTGCTACCGTAAGA100.15708451146716934No Hit
GCAAAAGTTCTCAGGGATGTGCGTTGATACCACTGCTTCCCATGTACTCT100.15708451146716934Clontech Universal Primer Mix Long (95% over 21bp)
GCAAAAGTTCTCAGGGATGTCTGCGTTGATACCACTGCTTCCCATGTACT90.1413760603204524Clontech Universal Primer Mix Long (95% over 24bp)
GGTGTTTGGGATGGAATGGTGGTCCACGCAGGTGGTTCGCAGAAGCAGCA90.1413760603204524No Hit
GCAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATACC90.1413760603204524No Hit
GTGCTGTCTCTCTTTGATGGACTCTGCGTTGATACCACTGCTTCCCATGT80.12566760917373546Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAA80.12566760917373546No Hit
GTGCTGTCTCTCTTTGATGGACACTGACGACATGGTTCTACAGGATTCGT70.10995915802701854No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACCCCATGTACTCT70.10995915802701854Clontech SMART CDS Primer II A (95% over 21bp)
GTGCTGTCTCTCTTTGATGGAATCTGCGTTGATACCACTGCTTCCCATGT70.10995915802701854Clontech Universal Primer Mix Long (95% over 23bp)
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGATACCCCCA70.10995915802701854No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCCTGCATAGACCA70.10995915802701854No Hit
GTGCCTCCTTCTCTTTTGTTGCTGCATAGACCAAGTCTCTGCTACCGTAA70.10995915802701854No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG70.10995915802701854No Hit
GTGCTGTCTCTCTTTGAGGGTCATCCCCAAGCAGCTGCGTTGATACCACT70.10995915802701854No Hit
GTGCTGTCTCTCTTTGATGGAATCAGCTCTTCTTCTTGGCCGGCATCTTG70.10995915802701854No Hit
GAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGAGAACTTGCGCCTGT70.10995915802701854No Hit
GTGCCTCCTTCTCTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAA70.10995915802701854No Hit
GTGCTGTCTCTCTTTGATGGAATCCAGGATGTCTTCCAGTTTCATATCCA70.10995915802701854No Hit
GTGCTGTCTCTCTTTGATGGTCCTCACTTTGCTGAACTTTTGCCTCCCAT70.10995915802701854No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTTGCGTTGATACCA70.10995915802701854No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTA70.10995915802701854No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA70.10995915802701854No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGGACCCCCAT70.10995915802701854No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)