FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25741
Total Bases3.7 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGGGTGGGGGGTGTTT746328.992657627908784No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA669426.00520570296414No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA375614.591507711433122No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA314512.217862553902336No Hit
GCAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGGGTGGGGGTGTTTG3291.2781166232858088No Hit
GCAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGGGTGGGGGGTGTTTG2010.7808554446214211No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGACTCCTGGTGCTGAAGGA1190.46229750203954784No Hit
GTGCCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTA1090.42344897245639257No Hit
GCAAAAGTTCTAGGGATGTCCTATTGCTAAGTGGGGGTGGGGGGTGTTTG730.2835942659570335No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA610.23697603045724722No Hit
GAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGGGTGGGGGGTGTTTG610.23697603045724722No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGCTGCAT590.22920632454061615No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGACTGCATAGACCAAGTCTCTG590.22920632454061615No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGACTGCATAGACCAAGTCTCTG580.22532147158230062No Hit
GCAAAAGTCTCAGGGATGTCCTATTGCTAAGTGGGGGTGGGGGGTGTTTG570.2214366186239851No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC540.20978205974903852No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA530.205897206790723No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG530.205897206790723No Hit
GTGCTGTCTCTCTTTGATGGATCGCTACAGGGCTCCTGGTGCTGAAGGAC520.20201235383240745No Hit
GCAAAAGTTTCAGGGATGTCCTATTGCTAAGTGGGGGTGGGGGGTGTTTG510.19812750087409192No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA510.19812750087409192No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG500.1942426479157764No Hit
GCAAAAGTTCTCAGGGATGTGAAGCACCGCCAGGTCCTTTGAGTTTTAAG500.1942426479157764No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA440.17093353016588322No Hit
CAAAAGTTCTCAGGGATGTCCTATTGCTAAGTGGGGGTGGGGGGTGTTTG400.1553941183326211No Hit
GTGCTGTCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAT400.1553941183326211No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA370.14373955945767453No Hit
GTGCTGTCTCTCTTTGATGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC350.13596985354104346No Hit
GCAAAAGTTCTCAGGGATGTCCAGCCAATCACTCGGGCGGACTGCATGCA320.12431529466609689No Hit
GCAAAAGTTCTCAGGATGTCCTATTGCTAAGTGGGGGTGGGGGGTGTTTG300.11654558874946584No Hit
GCAAAAGTTCTCGGGGATGTCCTATTGCTAAGTGGGGGTGGGGGGTGTTT280.10877588283283478No Hit
GTGCCTCCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAT280.10877588283283478No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG260.10100617691620373No Hit
GCAAAAGTTCCAGGGATGTCCTATTGCTAAGTGGGGGTGGGGGGTGTTTG260.10100617691620373No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG260.10100617691620373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)