FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21435
Total Bases3.1 Mbp
Sequences flagged as poor quality0
Sequence length35-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA854839.878703055749945No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA459521.436902262654538No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA216810.114299043620248No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC4312.0107301142990437No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC3361.5675297410776767No Hit
GTGCCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTA2441.138325169115932No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGCTGCATAGA1290.6018194541637508No Hit
GCAAAAGTTCTCAGGGATGTAACTCTGCGTTGATACCACTGCTTCCCATG1220.569162584557966Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT1130.5271751807790996No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT1120.522509913692559No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1050.489853044086774No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1030.4805225099136926No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT880.410543503615582Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT870.4058782365290413Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAT860.40121296944250057No Hit
GCAAAAGTTCTCAGGGATGCAACTCTGCGTTGATACCACTGCTTCCCATG830.38721716818287844Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC830.38721716818287844No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTAAAGGA670.3125728947982272No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA650.30324236062514576No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGACTGCATAGACCAAGTCTCTG650.30324236062514576No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGACTGCATAGACCAAGTCTCTG640.29857709353860507No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG530.24725915558665734No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC440.205271751807791No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG380.17728014928854677No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA350.16328434802892466No Hit
GTGCCTCCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAT350.16328434802892466No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG340.15861908094238394No Hit
GTGCTGTCTCTCTTTGATGGATCGCTACAGGGCTCCTGGTGCTGAAGGAC320.14928854676930253No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA280.13062747842313974No Hit
GGGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG260.12129694425005833No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC250.1166316771635176Clontech SMART CDS Primer II A (95% over 24bp)
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG250.1166316771635176No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG230.10730114299043621No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA230.10730114299043621No Hit
GTGCTGTCTCTCTTTGATGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC220.1026358759038955No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA220.1026358759038955No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)