FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15363
Total Bases2.2 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA948061.706697910564344No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA275917.958732018485975No Hit
GTGCCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTA2051.3343747965892079No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC1360.885243767493328No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1240.8071340233027403No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1160.7550608605090152No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGACTGCATAGACCAAGTCTCTG890.5793139360801927No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGACTGCATAGACCAAGTCTCTG810.5272407732864675No Hit
GTGCTGTCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAA640.4165853023498015No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG540.35149384885764495No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG520.3384755581592137No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA410.2668749593178416No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG380.24734752327019463No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA380.24734752327019463No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG370.24083837792097895No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGACTGCATAGACCAAGTCTCTG370.24083837792097895No Hit
GTGCCTCCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAA340.221310941873332No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG330.21480179652411638No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA320.20829265117490076No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAACA310.20178350582568508No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG290.1887652151272538No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGCTGCATAGA290.1887652151272538No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT270.17574692442882248No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA270.17574692442882248No Hit
GGGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG250.1627286337303912No Hit
GTGCTGTCTCTCTTTGATGGAATGCAGATGTACAGCCGTCAGCTGGCCTC240.15621948838117555No Hit
GTGCTGTCTCTCTTTGATGGATATGAAGCACCGCCAGGTCCTTTGAGTTT220.14320119768274425No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGTAGCA200.13018290698431295No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCGGGTGGTTCGCAGAAGCAGCA200.13018290698431295No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGTA200.13018290698431295No Hit
GTGCTGTCTCTCTTTGATGGACACTGACGACATGGTTCTACAGGATTCGT190.12367376163509732No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCCTGCATAGACCAAGTCTCT190.12367376163509732No Hit
GTGCTGTCTCTCTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAAT180.11716461628588166No Hit
GGTGTTTGGGATGGAATGGTGACCCACGCAGGTGGTTCGCAGAAGCAGCA180.11716461628588166No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA180.11716461628588166No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGACTGCATA170.110655470936666No Hit
GCAAAAGTTCTCAGGGATGTGGCGTATTAGCAACTCTGCGTTGATACCAC160.10414632558745038No Hit
GTGCTGTCTCTCTTTGATGGACAGGTGGCAGTTTGTGGGCCATCAGTGGT160.10414632558745038No Hit
CTCCAAGAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA160.10414632558745038No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)