FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12252
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA672054.84818805093046No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA189315.450538687561213No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA7756.325497877897486No Hit
GTGCCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTA1361.1100228534116878No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA1130.9222984002611819No Hit
GCAAAAGTTCTCAGGGATGTCCTCATCTTCCTGTTCATCATCGTCCTCAC1080.881488736532811No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC820.6692784851452824No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGCTGCAT740.6039830231798891No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGACTGCATAGACCAAGTCTCTG680.5550114267058439No Hit
GTGCTGTCTCTCTTTGATGGATCGCTACAGGGCTCCTGGTGCTGAAGGAC600.4897159647404506No Hit
GCAAAAGTTCTCAGGGATGTCAAAGATCAGATAGTTGTAGATATGCGTCA580.47339209924910214No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA520.4244205027750571No Hit
GTGCTGTCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAA440.35912504080966373No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGACTGCATAGACCAAGTCTCTG390.31831537708129287No Hit
GCAAAAGTTCTCAGGGATGTCCGGTAAGTGCCCTCATCCTGCCCTTTGGC380.3101534443356187No Hit
GTGCTGTCTCTCTTTGATGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC330.2693437806072478No Hit
GCAAAAGTTCTCAGGGATGTCCAAGATCAGATAGTTGTAGATATGCGTCA330.2693437806072478No Hit
GCAAAAGTTCTCAGGGATGTGAAGCACCGCCAGGTCCTTTGAGTTTTAAG260.21221025138752855No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG250.20404831864185438No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG240.1958863858961802No Hit
GCAAAAGTTCTCAGGGATGTCATCATCTTCCTGTTCATCATCGTCCTCAC230.18772445315050604No Hit
GCAAAAGTTCTCAGGGATGTCCCAGCGCTGCTCCCACTGCACCAGGCTCG220.17956252040483187No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACGGGGCTCCTGGTGCTGAAGGA220.17956252040483187No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCCTGCATAGACCA200.1632386549134835No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGACTGCATAGACCAAGTCTCTG200.1632386549134835No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGACTCCTGGTGCTGAAGGA190.15507672216780935No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGG180.14691478942213515No Hit
GTGCCTCCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAA180.14691478942213515No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTAGTGCTGAAGGA170.13875285667646098No Hit
GCAAAAGTTCTCAGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA160.1305909239307868No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGACTGCATA160.1305909239307868No Hit
GTGCTGTCTCTCTTTGATGGACACTGACGACATGGTTCTACAGGATTCGT150.12242899118511265No Hit
GTGCTGTCTCTCTTTATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC150.12242899118511265No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC150.12242899118511265No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA150.12242899118511265No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAGGGA150.12242899118511265No Hit
GCAAAAGTTCTCAGGGATGTCCGCGTCTGCTCGGCGGCTTCCCCTTTAAG150.12242899118511265No Hit
GTGCTGTCTCTCTTTGAGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC130.10610512569376428No Hit
GTGCTGTCTCTCTTTGTGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC130.10610512569376428No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCCTGCATAGACCAAG130.10610512569376428No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)