FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5590
Total Bases810.3 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA336660.21466905187835No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACCATCAAAGA2444.364937388193201No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGT1963.506261180679785No Hit
CAACCAAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTA1101.9677996422182469No Hit
GGGCAAAGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA661.180679785330948No Hit
CAACCAAAAGAGAAGGAGGCACCATCAAAGAGAGACAGCACGAATCCTGT621.10912343470483No Hit
CAACCAAAAGAGAAGGAGGCACCGACATCCCTGAGAACTTTTGCGAATCC601.073345259391771No Hit
CAACCAAAAGAGAAGGAGGCACCGAGCCTGGTGCAGTGGGAGCAGCGCTG591.0554561717352415No Hit
GGGCAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA470.8407871198568873No Hit
TCCTATCAGTGGCCGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC450.8050089445438283No Hit
GGGCAAAGGGTGAAGAGAAGGAGGGCAAATGAACAAAATGAAAGGAGGCA440.7871198568872988No Hit
GGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA370.6618962432915921No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA370.6618962432915921Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TCCTATCAGTGGCAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC340.6082289803220036No Hit
CAACCAAAAGAGAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGTA240.4293381037567084No Hit
GGGCAAAGGGTGAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA230.4114490161001789No Hit
GGGAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA220.3935599284436494No Hit
CAACCAAAAGAGAAGGAGGCACGAACAAGCATGGCACTCTTCATACGAAC220.3935599284436494No Hit
GGGCAAAGGGTAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA190.33989266547406083No Hit
GCATCAATCATCACAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCAC180.3220035778175313No Hit
CAACCAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTAG170.3041144901610018No Hit
GGGCAAAGGGTGAAGAGAAACAGCACCGTTACTTGGAGGAATCCTGTAGA170.3041144901610018No Hit
GCATCAATCATCACAGGGTTGTACTCTGTCACCATGTGGAGGCTGTTGAT160.28622540250447226No Hit
GGGCAAAGGGGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA150.26833631484794274No Hit
GGGCAAAGGGTGACAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGT150.26833631484794274No Hit
TCCTATCAGTGACAGCACCGTTACTTGGAGGAATCCTGTAGAACCATGTC140.25044722719141327No Hit
CAACCAAAAGAGAAGGAGGCACAGCCGTCATTACTGTCCTCCATGCCTTC140.25044722719141327No Hit
GGGCAAAGGGTGAAGAGACAGCACCGTTACTTGGAGGAATCCTGTAGAAC130.23255813953488372No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGGACAAAATGAAAGGAGGCA130.23255813953488372No Hit
TCCTATCAGTGGCCGCAAAGAGAGACAGCACCGTTACTTGGAGGAATCCT120.2146690518783542No Hit
TCCTATCAGTGGCCGCAAAGAGGGGAGAGGCCCTGGGCTTCACTTACTCT120.2146690518783542No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGTGC110.1967799642218247No Hit
GGGTAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA110.1967799642218247No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAGGGAGGCA100.17889087656529518No Hit
GGGCAAAGGGTGAGGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCA90.16100178890876565No Hit
GGGCAAAGGGTGAAGAGAAAGGGGGCAAATGAACAAAATGAAAGGAGGCA80.14311270125223613No Hit
GGGCAAAGGGTGAAGAGAGACAGCACCGTTACTTGGAGGAATCCTGTAGA80.14311270125223613No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGGGGCA80.14311270125223613No Hit
GGGCAAAGGGTGAAGAGAAAGAGGACAAATGAACAAAATGAAAGGAGGCA80.14311270125223613No Hit
CAACCAAAAGAGAAGGAGGGAAAAAGAAAGGGAAGGGGAAGAGGAAAGGA80.14311270125223613No Hit
CAATCAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTAG80.14311270125223613No Hit
GGGCAAAGGGTGAAGGAAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA80.14311270125223613No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGGAAGGAGGCA70.12522361359570663No Hit
CAACCAAAAGAGAAGGAGGCACGAATCCTGTAGAACCATGTCGTCAGGAT70.12522361359570663No Hit
CATCAAAGAGAGACAGCACGAATCCTGTAGAACCATGTCGTCAGTGTAGA70.12522361359570663No Hit
GGGCAAAGGGTGAAGAGAAGAGGGCAAATGAACAAAATGAAAGGAGGCAA70.12522361359570663No Hit
GCATCAATCATCACAGGGTTGGTTCGGCTGATCTGGCTGGCTAGGCGGGT70.12522361359570663No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC70.12522361359570663Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAAAGGGTGAAGAGAAAGAGGGCAAATGAACAAAATGAAAGGAGACA60.1073345259391771No Hit
GCATCAATCATCACAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA60.1073345259391771Clontech Universal Primer Mix Long (96% over 26bp)
GGGCAAAGGGTGAAGAGAAAGAGGGCGAATGAACAAAATGAAAGGAGGCA60.1073345259391771No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGCAAGTGAACAAAATGAAAGGAGGCA60.1073345259391771No Hit
GGGCAAAGGGTGAAGAGAAAGAGGGTAAATGAACAAAATGAAAGGAGGCA60.1073345259391771No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)