FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13908
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA636245.74345700316365No Hit
GTGCTGTCTCTCTTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGA297921.41932700603969No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA12368.886971527178602No Hit
GTGCCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTA1791.287029048029911No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC1471.0569456427955135No Hit
GTGCTGTCTCTCTTTGATGGAGAGGGTAAAGGGCTCCTGGTGCTGAAGGA1030.7405809605982169No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGACTGCATAGACCAAGTCTCTG1000.719010641357492No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG860.6183491515674432No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG860.6183491515674432No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC850.6111590451538682No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGACTGCATAGACCAAGTCTCTG770.5536381938452689No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGAAGCAACTCTG730.5248777681909692No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGCTGCATAGA450.32355478861087145No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG380.273224043715847No Hit
GTGCTGTCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAT340.24446361806154732No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG320.23008340523439746No Hit
GTGCTGTCTCTCTTGATGGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC320.23008340523439746No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGACTGCATAGACCAAGTCTCTG300.21570319240724764No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT280.20132297958009776No Hit
GCAAAAGTTCTCAGGGATGTGAAGCACCGCCAGGTCCTTTGAGTTTTAAG280.20132297958009776No Hit
GTGCCTCCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAT280.20132297958009776No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT260.18694276675294796Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGTCTCTCTTTGATGGATCGCTACAGGGCTCCTGGTGCTGAAGGAC250.179752660339373No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGACTGCATA250.179752660339373No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG240.1725625539257981No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA220.15818234109864826No Hit
GTGCTGTCTCTCTTTGATGAATCGCTACAGGGCTCCTGGTGCTGAAGGAC210.15099223468507333No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCCTGCATAGACCAAG200.14380212827149844No Hit
GTGCTGTCTCTCTTTGATGGACACTGACGACATGGTTCTACAGGATTCGT190.1366120218579235No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG190.1366120218579235No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCCTGCATAGACCA180.12942191544434856No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGCTGCAT180.12942191544434856No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA170.12223180903077366No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCCTGCATAGACCA160.11504170261719873No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGTAGCA150.10785159620362382No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA150.10785159620362382No Hit
GGGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG150.10785159620362382No Hit
GGTGTTTGGGATGGAATGGTGATCCGCGCAGGTGGTTCGCAGAAGCAGCA140.10066148979004888No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCGGCA140.10066148979004888No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA140.10066148979004888No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)