FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504
Total Bases217.8 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC25116.68882978723404No Hit
GTGCCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTA1318.710106382978724No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC916.050531914893617No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGCTGCATAGA754.986702127659575No Hit
GTGCTGTCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAA543.590425531914894No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT452.992021276595745No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGACTGCATAGACCAAGTCTCTG392.5930851063829787No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGCTGCAT352.327127659574468No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGACTGCATAGACCAAGTCTCTG322.127659574468085No Hit
GTGCCTCCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAA241.5957446808510638No Hit
GCAAAAGTTCTCAGGGATGTCCCTGCCGCTGATGTCCCCCTCCCTGTCTT181.196808510638298No Hit
GCAAAAGTTCTCAGGGATGTCCTGATCCAATATCGAGGTCGTAAACCCTA161.0638297872340425No Hit
CTCCAAGTAACGGTGCTGTTTCTCTTCACCCTTTGCCCCTGCATAGACCA140.9308510638297872No Hit
GTGCTGTCTCTCTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAAG140.9308510638297872No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTTGCGGCCACTGATAGGACTGCATA130.8643617021276596No Hit
GCAAAAGTTCTCAGGGATGTCCTCTGCGTTGATACCACTGCTTCCCATGT120.7978723404255319Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCAAAAGTTCTCAGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA120.7978723404255319No Hit
GTGCTGTCTCTCTTTGATGGAATCCTGACGACATGGTTCTACAGGATTCG120.7978723404255319No Hit
CTCCAAGTAACGGTGCTGTCACCCTTTGCCCCTGCATAGACCAAGTCTCT110.7313829787234043No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTGCGTTGATACCACT110.7313829787234043No Hit
GCAAAAGTTCTCAGGGATGTACTCTGCGTTGATACCACTGCTTCCCATGT90.598404255319149Clontech Universal Primer Mix Long (96% over 26bp)
GCAAAAGTTCTCAGGGATGTCGCGGTGAGTTCAGGCTTTTTCATGGTTTA90.598404255319149No Hit
GTGCTGTCTCTCTTTGATGGAATCGTATTCCGCTTCTAGAACAGTGTCCA90.598404255319149No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCAACCCTGTGATGATTGATGCCTG90.598404255319149No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGACTGCATAGACCAAGTCTCTG80.5319148936170213No Hit
GCAAAAGTTCTCAGGGATGTCTCTGCCGCTGATGTCCCCCTCCCTGTCTT80.5319148936170213No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTATC80.5319148936170213No Hit
GCAAAAGTTCTCAGGGATGTCCCCCATGTACTCTGCGTTGATACCACTGC70.4654255319148936Clontech SMART CDS Primer II A (95% over 24bp)
GCAAAAGTTCTCAGGGATGTCCCCAAGACCATATTCTATCCCTCCACTCT70.4654255319148936No Hit
GTGCTGTCTCTCTTTGATGGACACTGACGACATGGTTCTACAGGATTCGT60.39893617021276595No Hit
GTGCCTCCTTCTCTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAA60.39893617021276595No Hit
GCAAAAGTTCTCAGGGATGTCCACGCTTGTGGCTGTCATGGTGTGGAATG60.39893617021276595No Hit
GTGCTGTCTCTCTTTGATGCTGCATAGACCAAGTCTCTGCTACCGTAAGC60.39893617021276595No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGACCACC60.39893617021276595No Hit
CTCCAAGTAACGGTGCTGTCTCTTCACCCTTTGCCCCTGCATAGACCAAG60.39893617021276595No Hit
GTGCTGTCTCTCTTTGATGGATCATGAGTGGAAGTGTTGAGTGTCACATG50.3324468085106383No Hit
GTGCTGTCTCTCTTTGATGGGGTGCTGTCTCTCTTTGATGGGGTGCTGTC50.3324468085106383No Hit
GTGCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAA50.3324468085106383No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTA50.3324468085106383No Hit
GCAAAAGTTCTCAGGGATGTGAAGCACCGCCAGGTCCTTTGAGTTTTAAG50.3324468085106383No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTCTGCGTTGATACCA40.26595744680851063No Hit
GTGCTGTCTCTCTTTGATGGAATACTGACGACATGGTTCTACAGGATTCG40.26595744680851063No Hit
GTGCTGTCTCTCTTTGATGATGCTGTCTCTCTTTGATGGTGCTGTCTCTC40.26595744680851063No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGACTCTC40.26595744680851063No Hit
GTGCTGTCTCTCTTTGATGGCACTGACGACATGGTTCTACAGGATTCGTG40.26595744680851063No Hit
GCAAAAGTTCTCAGGGATGTCCAGGCTTTTTCATGGTTTAGTTCCTCACA40.26595744680851063No Hit
GCAAAAGTTCTCAGGGATGTCCGATGTCTTCACAACAGCCCTTCCCATAA30.19946808510638298No Hit
GCAAAAGTTCTCAGGGATGTCGAGAGCAGCCCCCTTTATCAATGCAGGAG30.19946808510638298No Hit
GTGCTGTCTCTCTTTGATGGAATTGTATTCCGCTTCTAGAACAGTGTCCA30.19946808510638298No Hit
GTGCCTCCTCTCTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAAG30.19946808510638298No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGATGTCTCTC30.19946808510638298No Hit
GTGCTGTCTCTCTTTGATGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC30.19946808510638298No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCGC30.19946808510638298No Hit
CTCCAAGTAACGGTGCTGTCTCTCTTCACCCTTTGCCCCTGCATAGACCA30.19946808510638298No Hit
GCAAAAGTTCTCAGGGATGTGATGATTGATGCCTGCATAGACCAAGTCTC30.19946808510638298No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTGGTTGCTGCATAGAC30.19946808510638298No Hit
GTGCTGTCTCTCTTTGATGGAATCCACACAGCCCTGTGTCCTCACAAGGC30.19946808510638298No Hit
GTGCTGTCTCTCTTTGATGGAATCGTGCCTCCTTCTCTTTTGGTTGCTGC30.19946808510638298No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGACCCCCATG30.19946808510638298No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTC30.19946808510638298No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGGACCCCATG20.13297872340425532No Hit
GTGCTGTCTCTCTTTGATTGCTGCATAGACCAAGTCTCTGCTACCGTAAG20.13297872340425532No Hit
GTGCCTCCTCTCTTTTGTTGCTGCATAGACCAAGTCTCTGCTACCGTAAG20.13297872340425532No Hit
GCAAAAGTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGCTGCATA20.13297872340425532No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGTGAGGGCCACC20.13297872340425532No Hit
GTGCTGTCTCTCTTTTGGTTCTGCATAGACCAAGTCTCTGCTACCGTAAG20.13297872340425532No Hit
GCAAAAGTTCTCAGGGATGTCCTTCTCTTTTGGTTGCTGCATAGACCAAG20.13297872340425532No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGATACCCCCA20.13297872340425532No Hit
CTCCAAGTAACGGTGCCGCCACTGATAGGACTGCATAGACCAAGTCTCTG20.13297872340425532No Hit
GCAAAAGTTCTCAGGGATGTCGCGGGTCATCACCGCGTCCGCGTACCCGG20.13297872340425532No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA20.13297872340425532No Hit
GCAAAAGTTCTCGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGCTGCATA20.13297872340425532No Hit
GTGCTGTCTCTCTTTGATGGAACTGTATTCCGCTTCTAGAACAGTGTCCA20.13297872340425532No Hit
GTGCTGTCTCTCTTTGATGGTGCCCCTTCTCTTTTGGTTGCTGCATAGAC20.13297872340425532No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGATACCCCCAT20.13297872340425532No Hit
GCAAAAGTTCTCAGGGATGTCGGGGCAAAGAGCTTCTCAAATATACGCAT20.13297872340425532No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATTGTGCTGTCTCTC20.13297872340425532No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGGCCCCCATG20.13297872340425532No Hit
GCAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATACC20.13297872340425532No Hit
GCAAAAGTTCTCAGGGATGTAAAATCACAGCCTGATGCCATTAGAAGATG20.13297872340425532No Hit
GCAAAAGTTCTCAGGGATGTCCTCCTCCACTGAGTGACCTTTTACTATGG20.13297872340425532No Hit
GCAAAAGTTCTCAGGGATGTCGGGTGCCCTGTGCCTCCTTCTCTTTTGGT20.13297872340425532No Hit
GTGCTGTCTCTCTTTGATGGATGCTGTGATGATTGATGCCTGCATAGACC20.13297872340425532No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGACTCTA20.13297872340425532No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCACTC20.13297872340425532No Hit
GTGCTGTCTCTCTTTGATGGTCATCCCCAAGCAGCTTAGGGGACCCATGT20.13297872340425532No Hit
GCAAAAGTTCTCAGGGATGTCCAGCGGCCACGATGATGTCGTGCGCCGCA20.13297872340425532No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTATCTC20.13297872340425532No Hit
GCAAAAGTTCTCAGGGATGTCCTCCTTCTCTTTTGGTTGCTGCATAGACC20.13297872340425532No Hit
GTGCTGTCTCTCTTTTGTTGCTGCATAGACCAAGTCTCTGCTACCGTAAG20.13297872340425532No Hit
GTGCTGTCTCTCTTTGATGCACTGACGACATGGTTCTACAGGATTCGTGC20.13297872340425532No Hit
GTGCTGTCTCTCTTTGATGGACCTAGGCTTGACCCATGTACTCTGCGTTG20.13297872340425532No Hit
GTGCTGTCTCTCTTTGATGGGTGCTGTCTCTCTTTGATGGGTGCTGTCTG20.13297872340425532No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)