FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008149627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008149627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17684
Total Bases2.5 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCA1048059.26261026916987No Hit
CTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGA392722.20651436326623No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCTGTCTCTC1871.0574530649174396No Hit
GGTGTTTGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1450.8199502375028275No Hit
GTGCCTCCTTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTA1190.6729246776747342No Hit
GGTGTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG1140.644650531553947No Hit
GCAAAAGTTCTCAGGGATGTCGGTGCCTCCTTCTCTTTTGGTTGCTGCAT910.5145894593983261No Hit
GTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG680.38452838724270527No Hit
CTCCAAGTACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA660.3732187287943904No Hit
GGTGTTTGGGATGGATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG630.3562542411219181No Hit
CTCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA570.3223252657769735No Hit
CTCCAAGTAACGGTGCGGCCACTGATAGGACTGCATAGACCAAGTCTCTG560.31667043655281607No Hit
GGTGTTTGGGATGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG540.30536077810450124No Hit
CTCCAAGTAACGGTGCTGCCACTGATAGGACTGCATAGACCAAGTCTCTG530.2997059488803438No Hit
GTGCTGTCTCTCTTTGATGGTGCTGTCTCTCTTTGATGGTGCCTCCTTCT470.26577697353539925No Hit
GTGCTGTCTCTCTTTTGGTTGCTGCATAGACCAAGTCTCTGCTACCGTAT470.26577697353539925No Hit
CTCCAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA450.25446731508708437No Hit
GTGCTGTCTCTCTTTGATGGTGCCTCCTTCTCTTTTGGTTGCTGCATAGA430.24315765663876948No Hit
TCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA370.20922868129382494No Hit
GGTGTTTGGGTGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG320.18095453517303778No Hit
GGTGTTTGGGAGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG300.16964487672472292No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCACAGAAGCAGCA300.16964487672472292No Hit
GGTTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG270.15268038905225062No Hit
CTCCAATAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA260.14702555982809318No Hit
GTGCTGTCTCTCTTTGATGGATATGAAGCACCGCCAGGTCCTTTGAGTTT240.13571590137977835No Hit
CTCCAAGTAACGGTGCTGTCACTGATAGGACTGCATAGACCAAGTCTCTG240.13571590137977835No Hit
CTCCAAGTAACGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA220.12440624293146346No Hit
CTCCAAGTAAGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAA220.12440624293146346No Hit
GCAAAAGTTCTCAGGGATGTGAGGACCACCCATGTACTCTGCGTTGATAC210.11875141370730603No Hit
GCAAAAGTTCTCAGGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTA210.11875141370730603No Hit
GGTGTTTGGGATGGAATGGTGATCCGCGCAGGTGGTTCGCAGAAGCAGCA210.11875141370730603No Hit
GGGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG210.11875141370730603No Hit
GGTGTTTGGGATGGAATGGTGGTCCACGCAGGTGGTTCGCAGAAGCAGCA200.1130965844831486No Hit
GGTGTTTGGGATGGAATGTGATCCACGCAGGTGGTTCGCAGAAGCAGCAG190.10744175525899118No Hit
GGTGTTTGGGATGGAATGGCGATCCACGCAGGTGGTTCGCAGAAGCAGCA190.10744175525899118No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGTAGCA180.10178692603483375No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCGGGTGGTTCGCAGAAGCAGCA180.10178692603483375No Hit
GGTGTTTGGGATGGAATGGTGATCCACGCAGGTGGTTCGCAGAAGCAGCG180.10178692603483375No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)